Lamin A/C Antibody (E-1): sc-376248
- Lamin A/C Antibody (E-1) is a mouse monoclonal IgG1 κ Lamin A/C antibody, cited in 280 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-29 at the N-terminus of Lamin A/C of human origin
- Lamin A/C Antibody (E-1) is recommended for detection of Lamin A and Lamin C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-Lamin A/C Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Lamin A/C Antibody (E-1) for WB and IHC(P) applications. These reagents are now offered in bundles with Lamin A/C Antibody (E-1) (see ordering information below).
Lamin A/C Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Lamin A/C protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Lamin A/C antibody (E-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Lamin A/C plays a crucial role in maintaining the structural integrity of the nuclear envelope, which is essential for proper cellular function and organization. Lamin A/C (E-1) antibody targets proteins located in the inner nuclear membrane that provide mechanical support to the nucleus, thereby influencing gene expression and cell cycle regulation. Disruption of Lamin A/C can lead to various diseases, including muscular dystrophies and premature aging syndromes, highlighting Lamin A/C′s importance in cellular health. Lamin A interacts with several proteins involved in nuclear organization and chromatin dynamics, further underscoring Lamin A/C′s significance in cellular processes. Nuclear Lamin A is cleaved by Mch2, while Lamin C, a splice variant of Lamin A, differs only at the carboxy-terminus, sharing the first 566 amino acids with Lamin A.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Lamin A/C Antibody (E-1) References:
- Lamin A/C proteins in the spermatid acroplaxome are essential in mouse spermiogenesis. | Shen, J., et al. 2014. Reproduction. 148: 479-87. PMID: 25118303
- Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage. | Gibbs-Seymour, I., et al. 2015. Aging Cell. 14: 162-9. PMID: 25645366
- Lamin A/C sustains PcG protein architecture, maintaining transcriptional repression at target genes. | Cesarini, E., et al. 2015. J Cell Biol. 211: 533-51. PMID: 26553927
- Expression of Lamin A/C in early-stage breast cancer and its prognostic value. | Alhudiri, IM., et al. 2019. Breast Cancer Res Treat. 174: 661-668. PMID: 30610489
- PCAF Involvement in Lamin A/C-HDAC2 Interplay during the Early Phase of Muscle Differentiation. | Santi, S., et al. 2020. Cells. 9: PMID: 32698523
- Lamin A/C missense variants: from discovery to functional validation. | Lazarte, J. and Hegele, RA. 2021. NPJ Genom Med. 6: 102. PMID: 34862397
- Matrix remodeling controls a nuclear lamin A/C-emerin network that directs Wnt-regulated stem cell fate. | Tang, Y., et al. 2022. Dev Cell. 57: 480-495.e6. PMID: 35150612
- The BAF A12T mutation disrupts lamin A/C interaction, impairing robust repair of nuclear envelope ruptures in Nestor-Guillermo progeria syndrome cells. | Janssen, A., et al. 2022. Nucleic Acids Res. 50: 9260-9278. PMID: 36039758
- Lamin A/C and Vimentin as a Coordinated Regulator during Amoeboid Migration in Microscale Confined Microenvironments. | Wang, YJ., et al. 2023. Nano Lett. 23: 6727-6735. PMID: 37459599
- Lamin A/C and PI(4,5)P2-A Novel Complex in the Cell Nucleus. | Escudeiro-Lopes, S., et al. 2024. Cells. 13: PMID: 38474363
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lamin A/C Antibody (E-1) | sc-376248 | 200 µg/ml | $322.00 | |||
Lamin A/C Antibody (E-1): m-IgG Fc BP-HRP Bundle | sc-529844 | 200 µg Ab; 10 µg BP | $361.00 | |||
Lamin A/C Antibody (E-1): m-IgGκ BP-HRP Bundle | sc-522892 | 200 µg Ab, 40 µg BP | $361.00 | |||
Lamin A/C Antibody (E-1) AC | sc-376248 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Lamin A/C Antibody (E-1) HRP | sc-376248 HRP | 200 µg/ml | $322.00 | |||
Lamin A/C Antibody (E-1) FITC | sc-376248 FITC | 200 µg/ml | $336.00 | |||
Lamin A/C Antibody (E-1) PE | sc-376248 PE | 200 µg/ml | $349.00 | |||
Lamin A/C Antibody (E-1) Alexa Fluor® 488 | sc-376248 AF488 | 200 µg/ml | $364.00 | |||
Lamin A/C Antibody (E-1) Alexa Fluor® 546 | sc-376248 AF546 | 200 µg/ml | $364.00 | |||
Lamin A/C Antibody (E-1) Alexa Fluor® 594 | sc-376248 AF594 | 200 µg/ml | $364.00 | |||
Lamin A/C Antibody (E-1) Alexa Fluor® 647 | sc-376248 AF647 | 200 µg/ml | $364.00 | |||
Lamin A/C Antibody (E-1) Alexa Fluor® 680 | sc-376248 AF680 | 200 µg/ml | $364.00 | |||
Lamin A/C Antibody (E-1) Alexa Fluor® 790 | sc-376248 AF790 | 200 µg/ml | $364.00 | |||
Lamin A/C (E-1) Neutralizing Peptide | sc-376248 P | 100 µg/0.5 ml | $69.00 |