Anti-Lamin A/C Antibody (636) recommended for detection of Lamin A and Lamin C of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Anti-Lamin A/C Antibody (636): sc-7292

Anti-Lamin A/C Antibody (636) is rated 4.9 out of 5 by 16.
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Datasheets

    • Lamin A/C Antibody (636) is a mouse monoclonal IgG2b κ, cited in 422 publications, provided at 200 µg/ml
    • raised against porcine lamin preparation
    • Anti-Lamin A/C Antibody (636) is recommended for detection of Lamin A and Lamin C of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Lamin A/C Antibody (636) is available conjugated to agarose for IP; and to phycoerythrin for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 594 for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-7292 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Lamin A/C (636): sc-7292.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for Lamin A/C Antibody (636) for WB and IHC(P) applications. These reagents are now offered in bundles with Lamin A/C Antibody (636) (see ordering information below).
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What is the difference in specificity and efficacy or advantage/disadvantage of this product (sc-7292) compared to the Anti-Lamin A/C Antibody (E-1): sc-376248?

Asked by: missy
Thank you for your question. Anti-Lamin A/C Antibody (636) is a mouse monoclonal IgG2b κ recommended for detection of Lamin A and Lamin C of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Anti-Lamin A/C Antibody (E-1) is a mouse monoclonal IgG1 κ recommended for detection of Lamin A and Lamin C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine. If you are intending to use one or the other for detection of Lamin A and C from mouse, rat, or human in WB, IP, IF, or IHC(P), either antibody would perform well and with very similar results.
Answered by: Tech Support Europe
Date published: 2021-11-08

Can this antibody be used to detect claeved Lamin A / C?

Asked by: Warner
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I used in the past Ab sc-376248 which is no longer available. I would like to test sc-7292 to verify if it is a valid substitute.

Asked by: GigiR
Thank you for your question. Our website was having technical issues today. sc-376248 is definitely still available and you can continue using this antibody. Please reach out to your distributor for information if you still would like to find out how to get a test sample of a different antibody.
Answered by: Tech Service
Date published: 2020-02-07

Can you send me the MSDS/SDS for the following product? Lamin A/C Antibody (636): sc-7292

Asked by: Lozinski
Thank you for your question. This antibody, sc-7292, Lamin A/C (636), is produced by Santa Cruz Biotechnology and is a non-hazardous products for which no SDS is required. Our primary antibodies are provided in a solution of 1x PBS, 0.1% sodium azide, and 0.1% gelatin. You can find the datasheet for this product by following the link below: https://datasheets.scbt.com/sc-7292.pdf Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-04-03

What is the recommended secondary reagent to be used with this mouse monoclonal primary for WB?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-16

I need to use an antibody to detect Lamin in porcine samples. Do you know if this mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2016-12-16
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Rated 5 out of 5 by from good for western I am glad I bought this a few weeks ago. I did western with it. the band is good.
Date published: 2022-02-23
Rated 3 out of 5 by from Not suitable for mouse IF I compared to other company's mouse mAb for IF of both MEF wt and MEF LmnA/C-/-. 636 is not capable of IF with mouse embryonic fibroblasts in our condition (4%PFA 15min fixation followed 1:500 dilution / 4C overnight reaction).
Date published: 2021-01-30
Rated 5 out of 5 by from Great signal It shows a great clean signal in western blot studies of nuclear extracts from rat liver samples
Date published: 2021-01-18
Rated 5 out of 5 by from Great bands Dillution 1/250 and it worked great in western blot!
Date published: 2019-04-01
Rated 5 out of 5 by from works very well Quality is great and the price is very good given that all the items needed. It worked well with 1:500 dilution factor and for whole cell and cytoplasmic lysis
Date published: 2019-03-10
Rated 5 out of 5 by from nice and clear signal It showed a clear and strong signal in WB of canine samples.
Date published: 2017-11-06
Rated 5 out of 5 by from work well work well in wb at 1:1000, HCT116 cells, SW480 cells
Date published: 2017-10-16
Rated 5 out of 5 by from Great signal I have used the Lamin A/C antibody for western blotting (1:500 dilutioin) and I get a good clean signal. I have used this for detection with nuclear extracts and cytoplasmic extracts and I get a clean signal in my nuclear extracts.
Date published: 2017-08-25
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