Date published: 2025-9-6

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Lamin A/C 항체 (636): sc-7292

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데이터 시트
  • Lamin A/C 항체(636) 는 마우스 monoclonal IgG2bκ, 468간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • porcine lamin preparation의하고 제작된다
  • 안티-Lamin A/C 항체 (636)는 WB, IP, IF, IHC(P) and FCM으로 mouse, rat and human유래의 Lamin A and Lamin C 를 감지하는 데에 추천한다.
  • Lamin A/C 항체(636)는 IP용으로 agarose에, IF, IHC(P), FCM용으로 phycoerythrin에 결합된 형태로 제공됩니다.
  • IF, IHC(P) and FCM에 사용하는Alexa Fluor® 594 접합 제품도 제공한다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • ChIP 에 사용하는 TransCruz시약(sc-7292 X, 200 µg/0.1 ml)이 있습니다.
  • Lamin A/C (636): sc-7292무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRPm-IgG2b BP-HRP 는 Lamin A/C 항체 (636) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Lamin A/C 항체(636) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

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Lamin A/C 항체(636)는 WB, IP, IF, IHC(P) 및 FCM에 의해 마우스, 래트 및 인간 기원의 Lamin A 및 Lamin C를 검출하는 IgG2b κ 마우스 단일 클론 Lamin A/C 항체이다. Lamin A/C 항체(636)는 공액 및 비공액 항-Lamin A/C 항체 형태로 모두 사용할 수 있다. 이전에 설명된 어떤 단백질분해효소 계열과도 서열, 구조 및 기질 특이성이 다른 독특한 시스테인 단백질분해효소 계열이 설명되었다. Ced-3/ICE라고 하는 이 계열은 ICE, CPP32, ICH-1/Nedd-2, Tx, Mch2, Mch3(ICE-LAP3 또는 CMH-1), Mch4 및 ICE-LAP6으로 구성된다. Ced-3/ICE 계열 구성체는 세포 사멸 기계의 핵심 구성 요소로 기능하고 세포 수명에 중요한 특정 표적 단백질을 파괴하는 작용을 한다. 핵 라민은 핵 외피와 세포 형태의 무결성을 유지하는 데 중요하다. 핵 라민 A는 Mch2에 의해 절단되지만 CPP32는 절단되지 않는다. 핵 라민 B는 ICE 계열의 미확인 구성체에 의한 세포자멸의 결과로 단편화된다. 라민 C는 Lamin A의 스플라이스 변형으로, 카르복시 말단에서만 다르다. 라민 A와 C는 처음 566개 아미노산에서 동일하며, Lamin C는 6개의 고유한 카르복시 말단 아미노산에서만 다르다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

Lamin A/C 항체 (636) 참고문헌:

  1. 뼈에서 중간엽줄기세포의 이동과 분화에서 라민 A/C의 중요한 역할.  |  Alcorta-Sevillano, N., et al. 2020. Cells. 9: PMID: 32466483
  2. 라민 A/C와 면역 체계: 하나의 중간 필라멘트, 다양한 얼굴.  |  Saez, A., et al. 2020. Int J Mol Sci. 21: PMID: 32854281
  3. 핵 외피와 중간 필라멘트 단백질 사이의 1차 및 2차 구조 모두에서 상동성이 있습니다.  |  McKeon, FD., et al. Nature. 319: 463-8. PMID: 3453101
  4. 핵 라민 A와 C의 cDNA 염기서열 분석 결과, 중간 필라멘트 단백질과 1차 및 2차 구조적 상동성이 밝혀졌습니다.  |  Fisher, DZ., et al. 1986. Proc Natl Acad Sci U S A. 83: 6450-4. PMID: 3462705
  5. Q353R-라민 A/C에 의한 TEAD1 트래핑은 확장성 심근증을 유발합니다.  |  Yamada, S., et al. 2023. Sci Adv. 9: eade7047. PMID: 37058558
  6. DNA 손상은 라미네이트 A/C의 ATR 매개 인산화를 통해 핵 외피 파열을 유도합니다.  |  Kovacs, MT., et al. 2023. Mol Cell. 83: 3659-3668.e10. PMID: 37832547
  7. 핵 라민의 동적 특성과 가능한 기능.  |  Moir, RD., et al. 1995. Int Rev Cytol. 162B: 141-82. PMID: 8557486
  8. 포유류의 새로운 포유류 상동 단백질인 ICE-LAP3는 Fas 및 종양 괴사인자 유도 세포 사멸 단백질 Ced-3의 새로운 포유류 상동 단백질로, Fas 및 종양 괴사인자 유도 세포 사멸 중에 활성화됩니다.  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
  9. ICE/Ced-3 유전자 계열의 새로운 구성원인 ICE-LAP6는 세포 독성 T 세포 프로테아제 과립분해효소 B에 의해 활성화됩니다.  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
  10. CPP32가 아닌 Mch2 알파에 의한 라민 A의 절단: 뚜렷한 기질 인식 특성을 가진 여러 인터루킨 1 베타 전환 효소 관련 프로테아제가 세포 사멸에서 활성화됩니다.  |  Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
  11. 두 개의 FADD 유사 도메인을 포함하는 새로운 인간 세포사멸 시스테인 프로테아제인 Mch4에 의한 CPP32 및 Mch3의 시험관 내 활성화.  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
  12. 라민 단백질 분해는 세포 사멸 중 핵 이벤트를 촉진합니다.  |  Rao, L., et al. 1996. J Cell Biol. 135: 1441-55. PMID: 8978814

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Lamin A/C 항체 (636)

sc-7292
200 µg/ml
$316.00

Lamin A/C (636): m-IgG Fc BP-HRP 번들

sc-525435
200 µg Ab; 10 µg BP
$354.00

Lamin A/C (636): m-IgG2b BP-HRP 번들

sc-549562
200 µg Ab; 10 µg BP
$354.00

Lamin A/C 항체 (636) X

sc-7292 X
200 µg/0.1 ml
$316.00

Lamin A/C 항체 (636) AC

sc-7292 AC
500 µg/ml, 25% agarose
$416.00

Lamin A/C 항체 (636) PE

sc-7292 PE
200 µg/ml
$343.00

Lamin A/C 항체 (636) Alexa Fluor® 546

sc-7292 AF546
200 µg/ml
$357.00

Lamin A/C 항체 (636) Alexa Fluor® 594

sc-7292 AF594
200 µg/ml
$357.00

Lamin A/C 항체 (636) Alexa Fluor® 680

sc-7292 AF680
200 µg/ml
$357.00

Lamin A/C 항체 (636) Alexa Fluor® 790

sc-7292 AF790
200 µg/ml
$357.00

would you be able to tell me if this antibody was raised against the full length porcine lamin preparation and where if known the specific region the antibody is recognizing? many thanks Steve

Asked by: Steve
Thank you for your question. sc-7292 Lamin A/C (636) has not been epitope mapped. Here is a link for more information on the protein. https://www.uniprot.org/uniprotkb/P02545/entry
Answered by: BlakeJ
Date published: 2025-07-28

Hello, does this antibody recognise N-terminal or C-terminal part of the protein? Best wishes

Asked by: npnp22
Thank you for your question. No epitope mapping has been performed for this antibody as of yet, so its binding site is not known at this point.
Answered by: Tech Support Europe
Date published: 2023-02-28

What is the difference in specificity and efficacy or advantage/disadvantage of this product (sc-7292) compared to the Anti-Lamin A/C Antibody (E-1): sc-376248?

Asked by: missy
Thank you for your question. Anti-Lamin A/C Antibody (636) is a mouse monoclonal IgG2b κ recommended for detection of Lamin A and Lamin C of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Anti-Lamin A/C Antibody (E-1) is a mouse monoclonal IgG1 κ recommended for detection of Lamin A and Lamin C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine. If you are intending to use one or the other for detection of Lamin A and C from mouse, rat, or human in WB, IP, IF, or IHC(P), either antibody would perform well and with very similar results.
Answered by: Tech Support Europe
Date published: 2021-11-08

Can this antibody be used to detect claeved Lamin A / C?

Asked by: Warner
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I used in the past Ab sc-376248 which is no longer available. I would like to test sc-7292 to verify if it is a valid substitute.

Asked by: GigiR
Thank you for your question. Our website was having technical issues today. sc-376248 is definitely still available and you can continue using this antibody. Please reach out to your distributor for information if you still would like to find out how to get a test sample of a different antibody.
Answered by: Tech Service
Date published: 2020-02-07

Can you send me the MSDS/SDS for the following product? Lamin A/C Antibody (636): sc-7292

Asked by: Lozinski
Thank you for your question. This antibody, sc-7292, Lamin A/C (636), is produced by Santa Cruz Biotechnology and is a non-hazardous products for which no SDS is required. Our primary antibodies are provided in a solution of 1x PBS, 0.1% sodium azide, and 0.1% gelatin. You can find the datasheet for this product by following the link below: https://datasheets.scbt.com/sc-7292.pdf Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-04-03

What is the recommended secondary reagent to be used with this mouse monoclonal primary for WB?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-16

I need to use an antibody to detect Lamin in porcine samples. Do you know if this mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2016-12-16
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Rated 5 out of 5 by from good for westernI am glad I bought this a few weeks ago. I did western with it. the band is good.
Date published: 2022-02-23
Rated 3 out of 5 by from Not suitable for mouse IFI compared to other company's mouse mAb for IF of both MEF wt and MEF LmnA/C-/-. 636 is not capable of IF with mouse embryonic fibroblasts in our condition (4%PFA 15min fixation followed 1:500 dilution / 4C overnight reaction).
Date published: 2021-01-30
Rated 5 out of 5 by from Great signalIt shows a great clean signal in western blot studies of nuclear extracts from rat liver samples
Date published: 2021-01-18
Rated 5 out of 5 by from Great bandsDillution 1/250 and it worked great in western blot!
Date published: 2019-04-01
Rated 5 out of 5 by from works very wellQuality is great and the price is very good given that all the items needed. It worked well with 1:500 dilution factor and for whole cell and cytoplasmic lysis
Date published: 2019-03-10
Rated 5 out of 5 by from nice and clear signalIt showed a clear and strong signal in WB of canine samples.
Date published: 2017-11-06
Rated 5 out of 5 by from work wellwork well in wb at 1:1000, HCT116 cells, SW480 cells
Date published: 2017-10-16
Rated 5 out of 5 by from Great signalI have used the Lamin A/C antibody for western blotting (1:500 dilutioin) and I get a good clean signal. I have used this for detection with nuclear extracts and cytoplasmic extracts and I get a clean signal in my nuclear extracts.
Date published: 2017-08-25
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Lamin A/C 항체 (636) is rated 4.9 out of 5 by 16.
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