Ku86 Antibody (5D5.8): sc-53021
- Ku86 Antibody (5D5.8) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against HeLa cell extracts
- recommended for detection of Ku86 of human origin by WB, IP and IF
- See Ku86 (B-1): sc-5280 for Ku86 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ku86 Antibody (5D5.8) for WB applications. These reagents are now offered in bundles with Ku86 Antibody (5D5.8) (see ordering information below).
Ku86 Antibody (5D5.8) is a mouse monoclonal IgG1 antibody that detects Ku86 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Ku86, also known as Ku80 or p80, is a crucial component of the Ku protein complex, which is primarily localized in the nucleus and consists of two subunits: Ku70 (p70) and Ku86 (p86). Anti-Ku86 antibody (5D5.8) recognizes a protein that plays a vital role in DNA repair and maintenance, particularly in the non-homologous end joining (NHEJ) pathway, which is essential for repairing double-strand breaks in DNA. Ku86 maintains genomic stability, as defects can lead to increased susceptibility to cancer and other genetic disorders. Additionally, Ku86 regulates transcription by interacting with various transcription factors and RNA polymerases, thereby influencing gene expression. Ku86′s ability to bind to DNA and facilitate repair protein recruitment underscores its significance in cellular responses to DNA damage, making anti-Ku86 antibody (5D5.8) an invaluable tool for researchers studying DNA repair mechanisms and related pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ku86 Antibody (5D5.8) References:
- Deletion of Ku86 causes early onset of senescence in mice. | Vogel, H., et al. 1999. Proc Natl Acad Sci U S A. 96: 10770-5. PMID: 10485901
- Human colon carcinoma cell-line HCT116 transfected by antisense cDNA as a tool to study the Ku86 involvement in cell proliferation. | Sadji, Z., et al. 2000. Cell Signal. 12: 745-50. PMID: 11152960
- Mammalian Ku86 protein prevents telomeric fusions independently of the length of TTAGGG repeats and the G-strand overhang. | Samper, E., et al. 2000. EMBO Rep. 1: 244-52. PMID: 11256607
- Mammalian Ku86 mediates chromosomal fusions and apoptosis caused by critically short telomeres. | Espejel, S., et al. 2002. EMBO J. 21: 2207-19. PMID: 11980718
- Role of human Ku86 in telomere length maintenance and telomere capping. | Jaco, I., et al. 2004. Cancer Res. 64: 7271-8. PMID: 15492246
- The lethality of Ku86 (XRCC5) loss-of-function mutations in human cells is independent of p53 (TP53). | Ghosh, G., et al. 2007. Radiat Res. 167: 66-79. PMID: 17214517
- Ku86 represses lethal telomere deletion events in human somatic cells. | Wang, Y., et al. 2009. Proc Natl Acad Sci U S A. 106: 12430-5. PMID: 19581589
- Serum anti-Ku86 is a potential biomarker for early detection of hepatitis C virus-related hepatocellular carcinoma. | Nomura, F., et al. 2012. Biochem Biophys Res Commun. 421: 837-43. PMID: 22554520
- PMA-induced dissociation of Ku86 from the promoter causes transcriptional up-regulation of histamine H(1) receptor. | Mizuguchi, H., et al. 2012. Sci Rep. 2: 916. PMID: 23209876
- Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants. | Errami, A., et al. 1996. Mol Cell Biol. 16: 1519-26. PMID: 8657125
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ku86 Antibody (5D5.8) | sc-53021 | 200 µg/ml | $322.00 | |||
Ku86 Antibody (5D5.8): m-IgG Fc BP-HRP Bundle | sc-536870 | 200 µg Ab; 10 µg BP | $361.00 | |||
Ku86 Antibody (5D5.8): m-IgGκ BP-HRP Bundle | sc-534038 | 200 µg Ab; 40 µg BP | $361.00 | |||
Ku86 Antibody (5D5.8): m-IgG1 BP-HRP Bundle | sc-544870 | 200 µg Ab; 20 µg BP | $361.00 |