KKIALRE Antibody (E-8): sc-515654
- KKIALRE Antibody (E-8) is a mouse monoclonal IgG1 κ KKIALRE antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 318-342 near the C-terminus of KKIALRE of human origin
- KKIALRE Antibody (E-8) is recommended for detection of KKIALRE p42 of human origin and Cdkl1 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-KKIALRE Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for KKIALRE Antibody (E-8) for WB applications. These reagents are now offered in bundles with KKIALRE Antibody (E-8) (see ordering information below).
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KKIALRE Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects KKIALRE protein of human origin and Cdkl1 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). KKIALRE (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KKIALRE protein plays a crucial role in cellular signaling pathways, particularly in MAP kinase regulation, which is essential for mediating responses to growth factors, hormones, and neurotransmitters. MAP kinases, specifically ERK 1 and ERK 2, are activated through dual phosphorylation at specific tyrosine and threonine residues, vital for cell proliferation and differentiation. KKIALRE is closely related to p56 KKIAMRE protein and is predominantly expressed in testis, kidney, brain, and lung, highlighting potential significance in these tissues. KKIALRE contains a conserved dual phosphorylation motif, Thr-Asp-Tyr, typically required for MAP kinase activation; however, EGF stimulation does not necessitate phosphorylation at this site, suggesting a distinct regulatory pathway.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
KKIALRE Antibody (E-8) References:
- Purification of a murine protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product: relationship to the fission yeast byr1 gene product. | Crews, CM. and Erikson, RL. 1992. Proc Natl Acad Sci U S A. 89: 8205-9. PMID: 1381507
- The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. | Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
- A family of human cdc2-related protein kinases. | Meyerson, M., et al. 1992. EMBO J. 11: 2909-17. PMID: 1639063
- Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies. | Boulton, TG. and Cobb, MH. 1991. Cell Regul. 2: 357-71. PMID: 1654126
- ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF. | Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
- Molecular cloning of the epidermal growth factor-stimulated protein kinase p56 KKIAMRE. | Taglienti, CA., et al. 1996. Oncogene. 13: 2563-74. PMID: 9000130
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KKIALRE Antibody (E-8) | sc-515654 | 200 µg/ml | $316.00 | |||
KKIALRE Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-531457 | 200 µg Ab; 10 µg BP | $354.00 | |||
KKIALRE Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-525149 | 200 µg Ab, 40 µg BP | $354.00 | |||
KKIALRE Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-544569 | 200 µg Ab; 20 µg BP | $354.00 | |||
KKIALRE Antibody (E-8) AC | sc-515654 AC | 500 µg/ml, 25% agarose | $416.00 | |||
KKIALRE Antibody (E-8) HRP | sc-515654 HRP | 200 µg/ml | $316.00 | |||
KKIALRE Antibody (E-8) FITC | sc-515654 FITC | 200 µg/ml | $330.00 | |||
KKIALRE Antibody (E-8) PE | sc-515654 PE | 200 µg/ml | $343.00 | |||
KKIALRE Antibody (E-8) Alexa Fluor® 488 | sc-515654 AF488 | 200 µg/ml | $357.00 | |||
KKIALRE Antibody (E-8) Alexa Fluor® 546 | sc-515654 AF546 | 200 µg/ml | $357.00 | |||
KKIALRE Antibody (E-8) Alexa Fluor® 594 | sc-515654 AF594 | 200 µg/ml | $357.00 | |||
KKIALRE Antibody (E-8) Alexa Fluor® 647 | sc-515654 AF647 | 200 µg/ml | $357.00 | |||
KKIALRE Antibody (E-8) Alexa Fluor® 680 | sc-515654 AF680 | 200 µg/ml | $357.00 | |||
KKIALRE Antibody (E-8) Alexa Fluor® 790 | sc-515654 AF790 | 200 µg/ml | $357.00 | |||
KKIALRE (E-8) Neutralizing Peptide | sc-515654 P | 100 µg/0.5 ml | $68.00 |