KIN17 Antibody (K58): sc-32769

KIN17 Antibody (K58) is a mouse monoclonal IgG2b kappa light chain antibody that detects KIN17 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). KIN17 (K58) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KIN17 plays a crucial role in the cellular response to DNA damage, specifically binding to bent or curved double-stranded DNA fragments that are often found at illegitimate recombination sites, which is vital for maintaining genomic stability. KIN17 is ubiquitously expressed, with the highest levels found in muscle, heart, and testis tissues, and KIN17 expression notably increases in response to low doses of ionizing radiation in mammalian cells. Additionally, KIN17 expression rises during hyperproliferative phases in keratinocytes and is further enhanced by UVC irradiation, contingent upon the presence of functional XPA and XPC proteins. Antisense studies have demonstrated that a reduction in KIN17 correlates with decreased cell proliferation and an accumulation of cells in early and mid-S phase, highlighting KIN17′s importance in cell cycle regulation. Furthermore, in SV40-transformed fibroblasts, KIN17 is overexpressed and interacts with the Large T antigen, which subsequently reduces T-antigen-dependent DNA replication. The gene encoding human KIN17 is located on chromosome 10p14, underscoring KIN17′s significance in both normal cellular function and the response to DNA damage.

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KIN17 Antibody (K58) References:

1. Molecular cloning and characterization of the human KIN17 cDNA encoding a component of the UVC response that is conserved among metazoans.  |  Kannouche, P., et al. 2000. Carcinogenesis. 21: 1701-10. PMID: 10964102
2. Ionizing radiation triggers chromatin-bound kin17 complex formation in human cells.  |  Biard, DS., et al. 2002. J Biol Chem. 277: 19156-65. PMID: 11880372
3. Human kin17 protein directly interacts with the simian virus 40 large T antigen and inhibits DNA replication.  |  Miccoli, L., et al. 2002. Cancer Res. 62: 5425-35. PMID: 12359749
4. Global genome repair is required to activate KIN17, a UVC-responsive gene involved in DNA replication.  |  Masson, C., et al. 2003. Proc Natl Acad Sci U S A. 100: 616-21. PMID: 12525703
5. Kin17, a mouse nuclear zinc finger protein that binds preferentially to curved DNA.  |  Mazin, A., et al. 1994. Nucleic Acids Res. 22: 4335-41. PMID: 7937163
6. KIN17, a mouse nuclear protein, binds to bent DNA fragments that are found at illegitimate recombination junctions in mammalian cells.  |  Mazin, A., et al. 1994. Mol Gen Genet. 244: 435-8. PMID: 8078469
7. Enhanced expression of the Kin17 protein immediately after low doses of ionizing radiation.  |  Biard, DS., et al. 1997. Radiat Res. 147: 442-50. PMID: 9092924
8. Differential expression of the HsKin17 protein during differentiation of in vitro reconstructed human skin.  |  Biard, DS., et al. 1997. Arch Dermatol Res. 289: 448-56. PMID: 9266022

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-KIN17 Antibody (K58) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-KIN17 Antibody (K58) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

KIN17 Antibody (K58) Specifications

Alternate Product Name: KIN17

Product Clone Name: K58

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: KIN17

Epitope Species: human

Product Reactivity/Detection: KIN17

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for KIN17 Antibody (K58): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent