KDEL ER Marker Antibody (10C3): sc-58774

KDEL ER Marker Antibody (10C3) is a mouse monoclonal IgG2a antibody that detects the KDEL ER Marker protein of broad species origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-KDEL ER Marker antibody (10C3) is available as the non-conjugated format. KDEL ER Marker protein plays a crucial role in the endoplasmic reticulum (ER) by ensuring proper retention of misfolded proteins, which is vital for maintaining cellular homeostasis. When proteins are misfolded, these can trigger ER stress response, leading to a cascade of cellular events aimed at restoring normal function. KDEL sequence, a C-terminal tetrapeptide retention signal (Lys-Asp-Glu-Leu), is essential for preventing secretion of misfolded proteins. KDEL sequence achieves this by binding to KDEL receptors, such as KDELR2, which cycle between Golgi apparatus and ER, facilitating retrieval of KDEL-tagged proteins back to ER. This mechanism is particularly important in research settings, as KDEL tags allow for coexpression of target proteins and subsequent redistribution to ER, providing insights into protein folding and trafficking processes.

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KDEL ER Marker Antibody (10C3) References:

1. The KDEL receptor mediates a retrieval mechanism that contributes to quality control at the endoplasmic reticulum.  |  Yamamoto, K., et al. 2001. EMBO J. 20: 3082-91. PMID: 11406585
2. The KDEL receptor modulates the endoplasmic reticulum stress response through mitogen-activated protein kinase signaling cascades.  |  Yamamoto, K., et al. 2003. J Biol Chem. 278: 34525-32. PMID: 12821650
3. Src regulates Golgi structure and KDEL receptor-dependent retrograde transport to the endoplasmic reticulum.  |  Bard, F., et al. 2003. J Biol Chem. 278: 46601-6. PMID: 12975382
4. A brefeldin A-like phenotype is induced by the overexpression of a human ERD-2-like protein, ELP-1.  |  Hsu, VW., et al. 1992. Cell. 69: 625-35. PMID: 1316805
5. Sequence of a second human KDEL receptor.  |  Lewis, MJ. and Pelham, HR. 1992. J Mol Biol. 226: 913-6. PMID: 1325562
6. The subcellular distribution of multigene family 110 proteins of African swine fever virus is determined by differences in C-terminal KDEL endoplasmic reticulum retention motifs.  |  Netherton, C., et al. 2004. J Virol. 78: 3710-21. PMID: 15016891
7. Regulation of Golgi signaling and trafficking by the KDEL receptor.  |  Cancino, J., et al. 2013. Histochem Cell Biol. 140: 395-405. PMID: 23873287
8. Crosstalk between KDEL receptor and EGF receptor mediates cell proliferation and migration via STAT3 signaling.  |  Jia, J., et al. 2024. Cell Commun Signal. 22: 140. PMID: 38378560
9. A luminal calcium-binding protein with a KDEL endoplasmic reticulum retention motif in the ER-Golgi intermediate compartment.  |  Schweizer, A., et al. 1993. Eur J Cell Biol. 60: 366-70. PMID: 8330633
10. Dictyostelium discoideum protein disulfide isomerase, an endoplasmic reticulum resident enzyme lacking a KDEL-type retrieval signal.  |  Monnat, J., et al. 1997. FEBS Lett. 418: 357-62. PMID: 9428745
11. Identification of a novel mammalian endoplasmic reticulum-resident KDEL protein using an EST database motif search.  |  Kimata, Y., et al. 2000. Gene. 261: 321-7. PMID: 11167020
12. Plant-derived mouse IgG monoclonal antibody fused to KDEL endoplasmic reticulum-retention signal is N-glycosylated homogeneously throughout the plant with mostly high-mannose-type N-glycans.  |  Triguero, A., et al. 2005. Plant Biotechnol J. 3: 449-57. PMID: 17173632

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-KDEL ER Marker Antibody (10C3) has 51 citations in a variety of scientific publications.

Primary antibodies like Anti-KDEL ER Marker Antibody (10C3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

KDEL ER Marker Antibody (10C3) Specifications

Alternate Product Name: Endoplasmic Reticulum Marker

Product Clone Name: 10C3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 649-654

Epitope Target: GRP 78

Epitope Species: rat

Product Reactivity/Detection: proteins containing the SEKDEL sequence in mammals, birds, insects and plants; recognises GPR78 and GPR94 with particular prominence

Species Reactivity/Detection: broad species

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 50 µg IgG_2a in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 51 citations in various scientific publications

Available Conjugates/Forms for KDEL ER Marker Antibody (10C3): N/A