KDEL ER Marker Antibody (10C3) recommended for detection of proteins containing the SEKDEL sequence in mammals, birds, insects and plants; recognises GPR78 and GPR94 with particular prominence of broad species origin by WB, IP & IF

KDEL ER Marker Antibody (10C3): sc-58774

KDEL ER Marker Antibody (10C3) is rated 4.9 out of 5 by 14.
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Datasheets

    • Anti-KDEL ER Marker Antibody (10C3) is a mouse monoclonal IgG2a KDEL ER Marker antibody provided at 100 µg/ml
    • raised against amino acids 649-654 of GRP 78 of rat origin
    • recommended for detection of proteins containing the SEKDEL sequence in mammals, birds, insects and plants; recognises GPR78 and GPR94 with particular prominence of broad species origin by WB, IP and IF
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of KDEL ER Marker (10C3): sc-58774.

Can this antibody be used for immunohistochemistry?

Asked by: BeatrizHG
Thank you for your question. We recommend this antibody for IF, or immunofluorescent staining of fresh or frozen tissues or cells fixed in acetone or methanol. There are many citations using this antibody for IF with PFA-fixation, as well as for ICC. There is one publication using the antibody for IHC, PMID: 28369137.
Answered by: Technical Support
Date published: 2018-12-23

I need to use an antibody to detect the protein in samples of non-mammalian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 9
Date published: 2017-12-10

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-12
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Rated 5 out of 5 by from Very good antibody We bought this antibody and we used it succesfuly on human endothelial cells line EA.hy926.
Date published: 2019-01-31
Rated 5 out of 5 by from WeternBlot We used KDEL ER Marker (10C3) antibody for WB in Mouse lung primary fibroblast, and it worked very well. We used anti-mouse IgG.
Date published: 2018-03-28
Rated 5 out of 5 by from Works great in IF I tried the anti KDEL antibody as ER marker in IF in FRTL-5 cells (rat cell line) and MDCK cells (dog cell line) and it worked perfectly fine!
Date published: 2018-02-23
Rated 4 out of 5 by from Sehr gute Signal am Western Blot Works very good for the western blot application for neurons from iPS. 1:1000
Date published: 2017-11-23
Rated 5 out of 5 by from Works great! Works great for WB in HeLa and MEFs. No background. I used 1:500.
Date published: 2017-11-03
Rated 5 out of 5 by from Works great in WB This KDEL ER Marker recognizes GPR78 and GPR94 in human liver and mouse liver by WB. Clean background and strong signal.
Date published: 2017-10-31
Rated 5 out of 5 by from Strong WB Very clear western blot data; strong signal with low background
Date published: 2017-01-31
Rated 5 out of 5 by from Good antibody Detects proteins with SEKDEL sequence. Works in mammals, birds, insects, and plants. Good for WB, IP, and IF.
Date published: 2017-01-27
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