KAT II Antibody (G-4): sc-377158
- KAT II Antibody (G-4) is a mouse monoclonal IgG2a κ KAT II antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 54-354 mapping within an internal region of KAT II of human origin
- KAT II Antibody (G-4) is recommended for detection of KAT II isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-KAT II Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for KAT II Antibody (G-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with KAT II Antibody (G-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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KAT II Antibody (G-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects KAT II protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). KAT II (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KAT II, a member of the kynurenine aminotransferase family, plays a crucial role in lysine degradation, which is essential for maintaining amino acid homeostasis and energy metabolism within cells. This mitochondrial protein is predominantly expressed in the liver but is also found in significant amounts in the heart, brain, kidney, pancreas, ovary, and testis. KAT II catalyzes the conversion of L-2-aminoadipate and 2-oxoglutarate into 2-oxoglutaramate and L-glutamate, a reaction that is vital for proper functioning of metabolic pathways. Notably, KAT II functions as a homodimer, which may influence enzymatic activity and interactions with other proteins, thereby impacting various physiological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
KAT II Antibody (G-4) References:
- Genomic organization and expression analysis of mouse kynurenine aminotransferase II, a possible factor in the pathophysiology of Huntington's disease. | Yu, P., et al. 1999. Mamm Genome. 10: 845-52. PMID: 10441733
- Some metabotropic glutamate receptor ligands reduce kynurenate synthesis in rats by intracellular inhibition of kynurenine aminotransferase II. | Battaglia, G., et al. 2000. J Neurochem. 75: 2051-60. PMID: 11032894
- L-cysteine sulphinate, endogenous sulphur-containing amino acid, inhibits rat brain kynurenic acid production via selective interference with kynurenine aminotransferase II. | Kocki, T., et al. 2003. Neurosci Lett. 346: 97-100. PMID: 12850557
- Biochemical and phenotypic abnormalities in kynurenine aminotransferase II-deficient mice. | Yu, P., et al. 2004. Mol Cell Biol. 24: 6919-30. PMID: 15282294
- Demonstration of kynurenine aminotransferases I and II and characterization of kynurenic acid synthesis in cultured cerebral cortical neurons. | Rzeski, W., et al. 2005. J Neurosci Res. 80: 677-82. PMID: 15880762
- Crystal structure of a human kynurenine aminotransferase II homologue from Pyrococcus horikoshii OT3 at 2.20 A resolution. | Chon, H., et al. 2005. Proteins. 61: 685-8. PMID: 16138312
- Demonstration of kynurenine aminotransferases I and II and characterization of kynurenic acid synthesis in oligodendrocyte cell line (OLN-93). | Wejksza, K., et al. 2005. Neurochem Res. 30: 963-8. PMID: 16258845
- Astrocytic localization of kynurenine aminotransferase II in the rat brain visualized by immunocytochemistry. | Guidetti, P., et al. 2007. Glia. 55: 78-92. PMID: 17024659
- Characterization of rat brain kynurenine aminotransferases I and II. | Guidetti, P., et al. 1997. J Neurosci Res. 50: 457-65. PMID: 9364331
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KAT II Antibody (G-4) | sc-377158 | 200 µg/ml | $316.00 | |||
KAT II Antibody (G-4): m-IgGκ BP-HRP Bundle | sc-523215 | 200 µg Ab, 40 µg BP | $354.00 | |||
KAT II Antibody (G-4) AC | sc-377158 AC | 500 µg/ml, 25% agarose | $416.00 | |||
KAT II Antibody (G-4) HRP | sc-377158 HRP | 200 µg/ml | $316.00 | |||
KAT II Antibody (G-4) FITC | sc-377158 FITC | 200 µg/ml | $330.00 | |||
KAT II Antibody (G-4) PE | sc-377158 PE | 200 µg/ml | $343.00 | |||
KAT II Antibody (G-4) Alexa Fluor® 488 | sc-377158 AF488 | 200 µg/ml | $357.00 | |||
KAT II Antibody (G-4) Alexa Fluor® 546 | sc-377158 AF546 | 200 µg/ml | $357.00 | |||
KAT II Antibody (G-4) Alexa Fluor® 594 | sc-377158 AF594 | 200 µg/ml | $357.00 | |||
KAT II Antibody (G-4) Alexa Fluor® 647 | sc-377158 AF647 | 200 µg/ml | $357.00 | |||
KAT II Antibody (G-4) Alexa Fluor® 680 | sc-377158 AF680 | 200 µg/ml | $357.00 | |||
KAT II Antibody (G-4) Alexa Fluor® 790 | sc-377158 AF790 | 200 µg/ml | $357.00 |