karyopherin β3 Antibody (A-2): sc-514122

karyopherin β3 Antibody (A-2) is a mouse monoclonal IgG2a antibody that detects karyopherin β3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Karyopherin β3 plays a crucial role in the nuclear import of proteins, particularly those that are part of the ribosomal assembly process. This protein is primarily located in the cytoplasm, where karyopherin β3 interacts with ribosomal proteins and facilitates their transport into the nucleus, ensuring that ribosome biogenesis occurs efficiently. The proper functioning of karyopherin β3 is vital for maintaining cellular homeostasis, as ribosomes are essential for protein synthesis, which is fundamental for cell growth and function. Disruptions in this transport mechanism can lead to various cellular dysfunctions and have been implicated in several diseases, including cancer. Anti-karyopherin β3 antibody (A-2) is an invaluable tool for researchers studying the dynamics of nuclear transport and ribosome assembly, providing insights into the molecular mechanisms underlying these critical biological processes.

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karyopherin β3 Antibody (A-2) References:

1. Nonstructural protein 5A of hepatitis C virus inhibits the function of karyopherin beta3.  |  Chung, KM., et al. 2000. J Virol. 74: 5233-41. PMID: 10799599
2. Crystallization and preliminary X-ray crystallographic studies of transportin 1 in complex with nucleocytoplasmic shuttling and nuclear localization fragments.  |  Imasaki, T., et al. 2006. Acta Crystallogr Sect F Struct Biol Cryst Commun. 62: 785-7. PMID: 16880557
3. Structural basis for substrate recognition and dissociation by human transportin 1.  |  Imasaki, T., et al. 2007. Mol Cell. 28: 57-67. PMID: 17936704
4. Karyopherin beta3: a new cellular target for the HPV-16 E5 oncoprotein.  |  Krawczyk, E., et al. 2008. Biochem Biophys Res Commun. 371: 684-8. PMID: 18455505
5. A glycine-rich domain of hnRNP H/F promotes nucleocytoplasmic shuttling and nuclear import through an interaction with transportin 1.  |  Van Dusen, CM., et al. 2010. Mol Cell Biol. 30: 2552-62. PMID: 20308327
6. FUS-NLS/Transportin 1 complex structure provides insights into the nuclear targeting mechanism of FUS and the implications in ALS.  |  Niu, C., et al. 2012. PLoS One. 7: e47056. PMID: 23056579
7. Transportin 1 accumulates in FUS inclusions in adult-onset ALS without FUS mutation.  |  Takeuchi, R., et al. 2013. Neuropathol Appl Neurobiol. 39: 580-4. PMID: 23362988
8. Cysteine (C)-x-C receptor 4 undergoes transportin 1-dependent nuclear localization and remains functional at the nucleus of metastatic prostate cancer cells.  |  Don-Salu-Hewage, AS., et al. 2013. PLoS One. 8: e57194. PMID: 23468933
9. A bimodular nuclear localization signal assembled via an extended double-stranded RNA-binding domain acts as an RNA-sensing signal for transportin 1.  |  Barraud, P., et al. 2014. Proc Natl Acad Sci U S A. 111: E1852-61. PMID: 24753571
10. Inhibition of the acetyltransferase NAT10 normalizes progeric and aging cells by rebalancing the Transportin-1 nuclear import pathway.  |  Larrieu, D., et al. 2018. Sci Signal. 11: PMID: 29970603

The preferred karyopherin β3 antibody is karyopherin β3 Antibody (B-7): sc-55527.