karyopherin β3 Antibody (B-7): sc-55527

karyopherin β3 Antibody (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects karyopherin beta 3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). karyopherin β3 (B-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. karyopherin beta 3 protein plays a crucial role in nuclear import of proteins, particularly those essential for ribosome biogenesis and cellular function. karyopherin beta 3 is primarily located in the cytoplasm and nucleus, where karyopherin beta 3 facilitates transport of specific substrates across the nuclear envelope. This localization ensures proteins required for critical cellular processes, such as gene expression and ribosome assembly, are efficiently delivered to the nucleus, maintaining cellular homeostasis and function. Additionally, karyopherin beta 3 has been implicated in import of certain ribosomal proteins, highlighting karyopherin beta 3′s importance in assembly of ribosomes, which are essential for protein synthesis. karyopherin beta 3′s ability to interact with various nuclear transport receptors underscores karyopherin beta 3′s significance in complex network of protein transport mechanisms that regulate cellular activities.

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karyopherin β3 Antibody (B-7) References:

1. Nonstructural protein 5A of hepatitis C virus inhibits the function of karyopherin beta3.  |  Chung, KM., et al. 2000. J Virol. 74: 5233-41. PMID: 10799599
2. Crystallization and preliminary X-ray crystallographic studies of transportin 1 in complex with nucleocytoplasmic shuttling and nuclear localization fragments.  |  Imasaki, T., et al. 2006. Acta Crystallogr Sect F Struct Biol Cryst Commun. 62: 785-7. PMID: 16880557
3. Karyopherin beta3: a new cellular target for the HPV-16 E5 oncoprotein.  |  Krawczyk, E., et al. 2008. Biochem Biophys Res Commun. 371: 684-8. PMID: 18455505
4. Transportin 1 accumulates in FUS inclusions in adult-onset ALS without FUS mutation.  |  Takeuchi, R., et al. 2013. Neuropathol Appl Neurobiol. 39: 580-4. PMID: 23362988
5. Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
6. Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.  |  Moroianu, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 2008-11. PMID: 7892216
7. Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import.  |  Lounsbury, KM., et al. 1996. J Biol Chem. 271: 2357-60. PMID: 8576188
8. The binding site of karyopherin alpha for karyopherin beta overlaps with a nuclear localization sequence.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 6572-6. PMID: 8692858
9. Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 7059-62. PMID: 8692944
10. Epstein-Barr virus nuclear antigen 1 forms a complex with the nuclear transporter karyopherin alpha2.  |  Fischer, N., et al. 1997. J Biol Chem. 272: 3999-4005. PMID: 9020106
11. Cloning and characterization of human karyopherin beta3.  |  Yaseen, NR. and Blobel, G. 1997. Proc Natl Acad Sci U S A. 94: 4451-6. PMID: 9114010
12. Karyopherin beta2 mediates nuclear import of a mRNA binding protein.  |  Bonifaci, N., et al. 1997. Proc Natl Acad Sci U S A. 94: 5055-60. PMID: 9144189

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-karyopherin β3 Antibody (B-7) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-karyopherin β3 Antibody (B-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

karyopherin β3 Antibody (B-7) Specifications

Alternate Product Name: Importin-5

Product Clone Name: B-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: karyopherin β3

Epitope Species: human

Product Reactivity/Detection: karyopherin β3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for karyopherin β3 Antibody (B-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin