karyopherin beta 3 Antibody (B-7) recommended for detection of karyopherin β3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

karyopherin β3 Antibody (B-7): sc-55527

karyopherin β3 Antibody (B-7) is rated 5.0 out of 5 by 5.
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Datasheets

    • Anti-karyopherin β3 Antibody (B-7) is a mouse monoclonal IgG1 (kappa light chain) karyopherin β3 antibody provided at 200 µg/ml
    • raised against amino acids 1-300 of karyopherin β3 of human origin
    • Anti-karyopherin beta 3 Antibody (B-7) is recommended for detection of karyopherin β3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-karyopherin beta 3 Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of karyopherin β3 (B-7): sc-55527.

What is the recommended fixation method for immunofluorescence staining with karyopherin β3 (B-7): sc-55527 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from very nice antibody The santa cruz offered me 15ul free antibody, I works very well.
Date published: 2017-11-26
Rated 5 out of 5 by from Worked great for western blotting in HeLa Worked great for western blotting in HeLa, Jurkat, HEL 92.1.7 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-06-08
Rated 5 out of 5 by from Strong Strong, clean bands in Western blot analysis of karyopherin 3 expression in NTERA-2 cl.D1 and A2058 whole cell lysates and HeLa and Jurkat nuclear extracts. -SCBT QC
Date published: 2015-06-06
Rated 5 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-03-11
Rated 5 out of 5 by from Beautiful immunofluorescence cytoplasmic Beautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-05
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