karyopherin β2 Antibody (D45): sc-32314

karyopherin β2 Antibody (D45) is a mouse monoclonal IgG1 kappa light chain antibody that detects karyopherin β2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Karyopherin β2 plays a crucial role in the nuclear transport system, specifically in the import of proteins into the nucleus, which is essential for regulating various cellular processes, including gene expression and cell cycle progression. Karyopherin β2 is primarily located in the cytoplasm and nucleus, where karyopherin β2 facilitates the transport of cargo proteins across the nuclear envelope. Proper localization of karyopherin β2 ensures proteins are delivered to the nucleus in a timely manner, maintaining cellular homeostasis and function. Disruptions in this transport mechanism can lead to various diseases, including cancer, where misregulation of nuclear proteins can result in uncontrolled cell growth. Karyopherin β2 also interacts with several other proteins, including karyopherin α1 and karyopherin β3, which are essential for recognition and docking of substrates at the nuclear pore complex. Anti-karyopherin β2 antibody (D45) serves as an invaluable tool for researchers studying nuclear transport mechanisms and their implications in health and disease.

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karyopherin β2 Antibody (D45) References:

1. Karyopherin-β2 Recognition of a PY-NLS Variant that Lacks the Proline-Tyrosine Motif.  |  Soniat, M. and Chook, YM. 2016. Structure. 24: 1802-1809. PMID: 27618664
2. Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains.  |  Guo, L., et al. 2018. Cell. 173: 677-692.e20. PMID: 29677512
3. Effect of nuclear import receptors on liquid-liquid phase separation.  |  Yoshizawa, T. and Matsumura, H. 2020. Biophys Physicobiol. 17: 25-29. PMID: 33110735
4. Mechanism of karyopherin-β2 binding and nuclear import of ALS variants FUS(P525L) and FUS(R495X).  |  Gonzalez, A., et al. 2021. Sci Rep. 11: 3754. PMID: 33580145
5. C9orf72-derived arginine-rich poly-dipeptides impede phase modifiers.  |  Nanaura, H., et al. 2021. Nat Commun. 12: 5301. PMID: 34489423
6. A minimal construct of nuclear-import receptor Karyopherin-β2 defines the regions critical for chaperone and disaggregation activity.  |  Fare, CM., et al. 2023. J Biol Chem. 299: 102806. PMID: 36529289
7. A new Karyopherin-β2 binding PY-NLS epitope of HNRNPH2 is linked to neurodevelopmental disorders.  |  Gonzalez, A., et al. 2023. bioRxiv.. PMID: 36711837
8. A new Karyopherin-β2 binding PY-NLS epitope of HNRNPH2 linked to neurodevelopmental disorders.  |  Gonzalez, A., et al. 2023. Structure. 31: 924-934.e4. PMID: 37279758
9. Karyopherin beta2 mediates nuclear import of a mRNA binding protein.  |  Bonifaci, N., et al. 1997. Proc Natl Acad Sci U S A. 94: 5055-60. PMID: 9144189
10. Distinct nuclear import and export pathways mediated by members of the karyopherin beta family.  |  Moroianu, J. 1998. J Cell Biochem. 70: 231-9. PMID: 9671229

The preferred karyopherin β2/2B antibody is karyopherin β2/2B Antibody (A-11): sc-365179.