karyopherin β2/2B Antibody (A-11): sc-365179

karyopherin β2/2B Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects karyopherin beta 2/2B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-karyopherin β2/2B antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. karyopherin beta 2/2B protein plays a crucial role in nuclear transport of proteins, specifically in import and export of RNA-binding proteins and mRNA. karyopherin β2/2B (A-11) monoclonal antibody is essential for maintaining cellular function and gene expression regulation, as karyopherin β2/2B facilitates transport of proteins like heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) into the nucleus, where they participate in RNA processing and transport. karyopherin β2B has been implicated in export of cellular mRNAs, highlighting its importance in post-transcriptional regulation of gene expression. The distinct substrate specificities of karyopherin β2 and karyopherin β2B, despite their high sequence identity, underscore the complexity of nuclear transport mechanisms and their implications in various cellular processes.

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karyopherin β2/2B Antibody (A-11) References:

1. Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp.  |  Chook, YM. and Blobel, G. 1999. Nature. 399: 230-7. PMID: 10353245
2. Karyopherin beta 2B participates in mRNA export from the nucleus.  |  Shamsher, MK., et al. 2002. Proc Natl Acad Sci U S A. 99: 14195-9. PMID: 12384575
3. Recognition of nuclear targeting signals by Karyopherin-β proteins.  |  Xu, D., et al. 2010. Curr Opin Struct Biol. 20: 782-90. PMID: 20951026
4. Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains.  |  Guo, L., et al. 2018. Cell. 173: 677-692.e20. PMID: 29677512
5. A new Karyopherin-β2 binding PY-NLS epitope of HNRNPH2 linked to neurodevelopmental disorders.  |  Gonzalez, A., et al. 2023. Structure. 31: 924-934.e4. PMID: 37279758
6. Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
7. Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.  |  Moroianu, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 2008-11. PMID: 7892216
8. Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import.  |  Lounsbury, KM., et al. 1996. J Biol Chem. 271: 2357-60. PMID: 8576188
9. The binding site of karyopherin alpha for karyopherin beta overlaps with a nuclear localization sequence.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 6572-6. PMID: 8692858
10. Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 7059-62. PMID: 8692944
11. Epstein-Barr virus nuclear antigen 1 forms a complex with the nuclear transporter karyopherin alpha2.  |  Fischer, N., et al. 1997. J Biol Chem. 272: 3999-4005. PMID: 9020106
12. Karyopherin beta2 mediates nuclear import of a mRNA binding protein.  |  Bonifaci, N., et al. 1997. Proc Natl Acad Sci U S A. 94: 5055-60. PMID: 9144189

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-karyopherin β2/2B Antibody (A-11) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-karyopherin β2/2B Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

karyopherin β2/2B Antibody (A-11) Specifications

Alternate Product Name: karyopherin β2/2B

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-29

Epitope Target: karyopherin β2

Epitope Species: human

Product Reactivity/Detection: karyopherin β2 and karyopherin β2B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for karyopherin β2/2B Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin