JNK1 Antibody (F-3): sc-1648

JNK1 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect JNK1, also known as MAPK8 or SAPK1, across various applications including WB, IP, IF, and IHCP. JNK1 mouse monoclonal antibody (F-3) is raised against amino acids 1-384 of the full-length human JNK1 protein, ensuring reliable recognition of all JNK1 p46 and p54 isoforms in mouse, rat, and human samples. JNK1 is a crucial member of the c-Jun N-terminal kinase (JNK) family within the mitogen-activated protein kinase (MAPK) pathway, playing a central role in regulating cellular responses to stress by phosphorylating and activating the transcription factor c-Jun, which is essential for cell proliferation, differentiation, and apoptosis. The function of JNK1 is vital for immune responses, including the differentiation of CD4+ T cells into TH1 and TH2 effector cells, and JNK1 forms protein complexes with molecules like c-Jun and Bcl-3 to modulate apoptotic pathways. Post-translational modifications of JNK1, particularly phosphorylation, are critical for activation and subsequent signaling activities, which are necessary for maintaining cellular homeostasis and responding to environmental stressors. Dysregulation of JNK1 has been implicated in various cancers such as hepatocellular carcinoma, breast cancer, and colorectal cancer, where JNK1 contributes to tumor development through mechanisms like altered histone methylation and other epigenetic modifications. JNK1 interacts with other signaling proteins such as MEKK1 and p38, further influencing apoptosis and cell survival. The human JNK1 gene is located on chromosome 10q11.22 and shares significant homology with JNK2, highlighting JNK1′s importance in both normal physiological functions and oncogenic processes.

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JNK1 Antibody (F-3) References:

1. Inhibition of c-Jun N-terminal kinase 2 expression suppresses growth and induces apoptosis of human tumor cells in a p53-dependent manner.  |  Potapova, O., et al. 2000. Mol Cell Biol. 20: 1713-22. PMID: 10669748
2. JNK is required for effector T-cell function but not for T-cell activation.  |  Dong, C., et al. 2000. Nature. 405: 91-4. PMID: 10811224
3. Isoforms of Jun kinase are differentially expressed and activated in human monocyte/macrophage (THP-1) cells.  |  Dreskin, SC., et al. 2001. J Immunol. 166: 5646-53. PMID: 11313405
4. Induced proliferation of human MRC-5 cells by nitrogen oxides via direct and indirect activation of MEKK1, JNK, and p38 signals.  |  Chou, FP., et al. 2002. Toxicol Appl Pharmacol. 181: 203-8. PMID: 12079429
5. Differential gene regulation by specific gain-of-function JNK1 proteins expressed in Swiss 3T3 fibroblasts.  |  Han, SY., et al. 2002. J Biol Chem. 277: 47167-74. PMID: 12354774
6. Roles of JNK-1 and p38 in selective induction of apoptosis by capsaicin in ras-transformed human breast epithelial cells.  |  Kang, HJ., et al. 2003. Int J Cancer. 103: 475-82. PMID: 12478662
7. JNK2 contains a specificity-determining region responsible for efficient c-Jun binding and phosphorylation.  |  Kallunki, T., et al. 1994. Genes Dev. 8: 2996-3007. PMID: 8001819
8. Defective T cell differentiation in the absence of Jnk1.  |  Dong, C., et al. 1998. Science. 282: 2092-5. PMID: 9851932

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JNK1 Antibody (F-3) has 196 citations in a variety of scientific publications.

Primary antibodies like Anti-JNK1 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JNK1 Antibody (F-3) Specifications

Alternate Product Name: JNK1

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-384

Epitope Target: JNK1 p46

Epitope Species: human

Product Reactivity/Detection: all JNK1 p46 and p54 isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 196 citations in various scientific publications

Available Conjugates/Forms for JNK1 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, TRITC: Rhodamine

The preferred p-JNK antibody is p-JNK Antibody (G-7): sc-6254.