JMJD2D Antibody (F-7): sc-393750
- JMJD2D Antibody (F-7) is a mouse monoclonal IgG1 κ JMJD2D antibody provided at 200 µg/ml
- raised against amino acids 341-510 mapping at the C-terminus of JMJD2D of mouse origin
- JMJD2D Antibody (F-7) is recommended for detection of JMJD2D of mouse and rat origin by WB, IP, IF and ELISA
- Anti-JMJD2D Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-393750 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for JMJD2D Antibody (F-7) for WB applications. These reagents are now offered in bundles with JMJD2D Antibody (F-7) (see ordering information below).
JMJD2D Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects JMJD2D of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). JMJD2D (F-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. JMJD2D, also known as Jumonji domain-containing protein 2D, JHDM3D, or KDM4D, is a 520 amino acid protein that plays a pivotal role in gene expression regulation through histone demethylase function. Located primarily in the nucleus, JMJD2D specifically removes methyl groups from Histone H3, essential for dynamic regulation of chromatin structure and function, thereby influencing transcriptional activity. JMJD2D′s ability to bind iron as a cofactor and possession of both JMJC and JMJN domains are critical for enzymatic activity during chromatin remodeling processes. Additionally, JMJD2D interacts with the ligand-bound form of androgen receptor (AR), enhancing AR expression, which is particularly significant since AR overexpression links to prostate cancer. This interaction suggests JMJD2D may influence carcinogenesis through AR regulation, making JMJD2D a valuable target for cancer biology research and therapeutic development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
JMJD2D Antibody (F-7) References:
- Identification and characterization of JMJD2 family genes in silico. | Katoh, M. and Katoh, M. 2004. Int J Oncol. 24: 1623-8. PMID: 15138608
- Reversal of histone lysine trimethylation by the JMJD2 family of histone demethylases. | Whetstine, JR., et al. 2006. Cell. 125: 467-81. PMID: 16603238
- Diversity within the JMJD2 histone demethylase family. | Shin, S. and Janknecht, R. 2007. Biochem Biophys Res Commun. 353: 973-7. PMID: 17207460
- Activation of androgen receptor by histone demethylases JMJD2A and JMJD2D. | Shin, S. and Janknecht, R. 2007. Biochem Biophys Res Commun. 359: 742-6. PMID: 17555712
- Comparative integromics on JMJD2A, JMJD2B and JMJD2C: preferential expression of JMJD2C in undifferentiated ES cells. | Katoh, Y. and Katoh, M. 2007. Int J Mol Med. 20: 269-73. PMID: 17611647
- Inflammation-induced JMJD2D promotes colitis recovery and colon tumorigenesis by activating Hedgehog signaling. | Zhuo, M., et al. 2020. Oncogene. 39: 3336-3353. PMID: 32094404
- Demethylase-independent function of JMJD2D as a novel antagonist of p53 to promote Liver Cancer initiation and progression. | Li, M., et al. 2020. Theranostics. 10: 8863-8879. PMID: 32754284
- Histone demethylase JMJD2D promotes the self-renewal of liver cancer stem-like cells by enhancing EpCAM and Sox9 expression. | Deng, Y., et al. 2021. J Biol Chem. 296: 100121. PMID: 33434575
- Demethylase JMJD2D induces PD-L1 expression to promote colorectal cancer immune escape by enhancing IFNGR1-STAT3-IRF1 signaling. | Chen, Q., et al. 2022. Oncogene. 41: 1421-1433. PMID: 35027670
- Histone Demethylase JMJD2D: A Novel Player in Colorectal and Hepatocellular Cancers. | Chen, Q., et al. 2022. Cancers (Basel). 14: PMID: 35740507
- JMJD2D stabilises and cooperates with HBx protein to promote HBV transcription and replication. | Kong, X., et al. 2023. JHEP Rep. 5: 100849. PMID: 37701334
- Methylation of the epigenetic JMJD2D protein by SET7/9 promotes prostate tumorigenesis. | Gu, R., et al. 2023. Front Oncol. 13: 1295613. PMID: 38045004
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JMJD2D Antibody (F-7) | sc-393750 | 200 µg/ml | $322.00 | |||
JMJD2D Antibody (F-7): m-IgG Fc BP-HRP Bundle | sc-530568 | 200 µg Ab; 10 µg BP | $361.00 | |||
JMJD2D Antibody (F-7): m-IgGκ BP-HRP Bundle | sc-524074 | 200 µg Ab, 40 µg BP | $361.00 | |||
JMJD2D Antibody (F-7) X | sc-393750 X | 200 µg/0.1 ml | $322.00 | |||
JMJD2D Antibody (F-7) AC | sc-393750 AC | 500 µg/ml, 25% agarose | $424.00 | |||
JMJD2D Antibody (F-7) HRP | sc-393750 HRP | 200 µg/ml | $322.00 | |||
JMJD2D Antibody (F-7) FITC | sc-393750 FITC | 200 µg/ml | $336.00 | |||
JMJD2D Antibody (F-7) PE | sc-393750 PE | 200 µg/ml | $349.00 | |||
JMJD2D Antibody (F-7) Alexa Fluor® 488 | sc-393750 AF488 | 200 µg/ml | $364.00 | |||
JMJD2D Antibody (F-7) Alexa Fluor® 546 | sc-393750 AF546 | 200 µg/ml | $364.00 | |||
JMJD2D Antibody (F-7) Alexa Fluor® 594 | sc-393750 AF594 | 200 µg/ml | $364.00 | |||
JMJD2D Antibody (F-7) Alexa Fluor® 647 | sc-393750 AF647 | 200 µg/ml | $364.00 | |||
JMJD2D Antibody (F-7) Alexa Fluor® 680 | sc-393750 AF680 | 200 µg/ml | $364.00 | |||
JMJD2D Antibody (F-7) Alexa Fluor® 790 | sc-393750 AF790 | 200 µg/ml | $364.00 |