JAM-C Antibody (A-4): sc-515893

JAM-C Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects JAM-C protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). JAM-C (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The junctional adhesion molecule (JAM) family, which includes JAM-C, plays a critical role in maintaining tight junction integrity in epithelial and endothelial cells, thereby regulating paracellular permeability and cell signaling. JAM-C is particularly important for transendothelial migration of leukocytes, a process essential for immune response and inflammation. JAM-C is constitutively expressed on various immune cells, including circulating monocytes, neutrophils, and lymphocyte subsets, facilitating their adhesion and migration during immune surveillance. Notably, JAM-C interacts with JAM-B, suggesting a functional relationship that may influence T cell trafficking and inflammatory response. JAM-C′s ability to modulate leukocyte extravasation highlights its significance in both normal immune function and pathological conditions, making anti-JAM-C antibody (A-4) a valuable tool for researchers studying cell adhesion mechanisms and immune responses.

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JAM-C Antibody (A-4) References:

1. Cutting edge: combined treatment of TNF-alpha and IFN-gamma causes redistribution of junctional adhesion molecule in human endothelial cells.  |  Ozaki, H., et al. 1999. J Immunol. 163: 553-7. PMID: 10395639
2. Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1.  |  Ebnet, K., et al. 2000. J Biol Chem. 275: 27979-88. PMID: 10856295
3. Homophilic interaction of junctional adhesion molecule.  |  Bazzoni, G., et al. 2000. J Biol Chem. 275: 30970-6. PMID: 10913139
4. Junctional adhesion molecule (JAM) is phosphorylated by protein kinase C upon platelet activation.  |  Ozaki, H., et al. 2000. Biochem Biophys Res Commun. 276: 873-8. PMID: 11027562
5. The molecular organization of endothelial junctions and their functional role in vascular morphogenesis and permeability.  |  Dejana, E., et al. 2000. Int J Dev Biol. 44: 743-8. PMID: 11061439
6. Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor.  |  Arrate, MP., et al. 2001. J Biol Chem. 276: 45826-32. PMID: 11590146
7. Vascular endothelial-junctional adhesion molecule (VE-JAM)/JAM 2 interacts with T, NK, and dendritic cells through JAM 3.  |  Liang, TW., et al. 2002. J Immunol. 168: 1618-26. PMID: 11823489
8. JAM2 interacts with alpha4beta1. Facilitation by JAM3.  |  Cunningham, SA., et al. 2002. J Biol Chem. 277: 27589-92. PMID: 12070135
9. Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration.  |  Johnson-Léger, CA., et al. 2002. Blood. 100: 2479-86. PMID: 12239159
10. Schwann cell-specific JAM-C-deficient mice reveal novel expression and functions for JAM-C in peripheral nerves.  |  Colom, B., et al. 2012. FASEB J. 26: 1064-76. PMID: 22090315
11. Dynamic trafficking and turnover of JAM-C is essential for endothelial cell migration.  |  Kostelnik, KB., et al. 2019. PLoS Biol. 17: e3000554. PMID: 31790392
12. Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration.  |  Martìn-Padura, I., et al. 1998. J Cell Biol. 142: 117-27. PMID: 9660867

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JAM-C Antibody (A-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-JAM-C Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JAM-C Antibody (A-4) Specifications

Alternate Product Name: JAM-C

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 32-310

Epitope Target: JAM-C

Epitope Species: human

Product Reactivity/Detection: JAM-C

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for JAM-C Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin