Date published: 2025-9-5
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JAK2 Antibody (C-10) - Western Blotting - Image 320285
JAK2 抗体 (C-10): sc-390539. HEL 92. 1. 7 (A) 和 MEG-01 (B) 全细胞裂解液中 JAK2 表达的 Western 印迹分析.
JAK2 抗体 (C-10): sc-390539. HEL 92. 1. 7 (A) 和 MEG-01 (B) 全细胞裂解液中 JAK2 表达的 Western 印迹分析.

JAK2 抗体 (C-10): sc-390539

4.5(28)
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说明书
  • JAK2 抗体 C-10 是小鼠单克隆 IgG2b κ，JAK2抗体, 在53篇文献中引用，规格为200 µg/ml
  • 与mouse的752-780氨基酸残基的JAK2
  • JAK2 抗体 (C-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 JAK2
  • 抗JAK2抗体（C-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取JAK2 (C-10): sc-390539的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是JAK2 Antibody (C-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与JAK2 Antibody (C-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外JAK抗体，包括JAK1, JAK2和JAK3

JAK2抗体（C-10）是一种IgG2b κ小鼠单克隆JAK2抗体（也称为酪氨酸蛋白激酶JAK2抗体、JAK-2抗体或Janus激酶2抗体），可通过WB、IP、IF和ELISA检测来自小鼠、大鼠和人类的JAK2蛋白。 JAK2抗体（C-10）既有非偶联抗JAK2抗体形式，也有多种偶联形式的抗JAK2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。JAK2（Janus激酶2）属于新兴的非受体Janus酪氨酸激酶家族，在淋巴造血系统中调节活化细胞因子受体下游的一系列细胞功能。免疫学刺激物，如干扰素和细胞因子，诱导Stat转录因子募集到细胞因子受体相关的JAK2。然后，JAK2使近端Stat因子磷酸化，随后这些因子二聚化，易位至细胞核并与靶基因启动子上游的cis元件结合以调节转录。经典的JAK / Stat途径对于通过刺激增殖，分化，存活和宿主对病原体的抵抗力来维持正常的免疫系统是必不可少的。改变JAK / Stat信号传导以减少细胞因子诱导的促炎反应代表了抗炎治疗的有吸引力的目标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

JAK2 抗体 (C-10) 参考文献:

  1. 细胞因子 II 类受体复合物眼中的 Jak-Stat 信号转导途径。  |  Kotenko, SV. and Pestka, S. 2000. Oncogene. 19: 2557-65. PMID: 10851054
  2. 糖皮质激素抑制 IL-2 诱导的 Jak-STAT 信号传导  |  Bianchi, M., et al. 2000. Proc Natl Acad Sci U S A. 97: 9573-8. PMID: 10920190
  3. 激活 JAK/STAT 通路可在大鼠急性心肌梗死中传递细胞保护信号。  |  Negoro, S., et al. 2000. Cardiovasc Res. 47: 797-805. PMID: 10974228
  4. 血管活性肠肽和垂体腺苷酸环化酶激活多肽抑制 IFN-gamma 在巨噬细胞中诱导的破伤风激酶-1-STAT1 激活。  |  Delgado, M. and Ganea, D. 2000. J Immunol. 165: 3051-7. PMID: 10975815
  5. Jak-STAT 通路：从细胞膜到细胞核的特异信号转导。  |  Heim, MH. 1996. Eur J Clin Invest. 26: 1-12. PMID: 8682149
  6. Jaks, Stats 和免疫系统。  |  Decker, T. and Meinke, A. 1997. Immunobiology. 198: 99-111. PMID: 9442382
  7. Jaks 和 STAT：生物学意义。  |  Leonard, WJ. and O'Shea, JJ. 1998. Annu Rev Immunol. 16: 293-322. PMID: 9597132

订购信息

产品名称产品编号规格价格数量收藏夹

JAK2 抗体 (C-10)

sc-390539
200 µg/ml
$316.00

JAK2 (C-10): m-IgGκ BP-HRP 套装

sc-523574
200 µg Ab, 40 µg BP
$354.00

JAK2 (C-10): m-IgG2b BP-HRP 套装

sc-549127
200 µg Ab; 10 µg BP
$354.00

JAK2 抗体 (C-10) AC

sc-390539 AC
500 µg/ml, 25% agarose
$416.00

JAK2 抗体 (C-10) HRP

sc-390539 HRP
200 µg/ml
$316.00

JAK2 抗体 (C-10) FITC

sc-390539 FITC
200 µg/ml
$330.00

JAK2 抗体 (C-10) PE

sc-390539 PE
200 µg/ml
$343.00

JAK2 抗体 (C-10) Alexa Fluor® 488

sc-390539 AF488
200 µg/ml
$357.00

JAK2 抗体 (C-10) Alexa Fluor® 546

sc-390539 AF546
200 µg/ml
$357.00

JAK2 抗体 (C-10) Alexa Fluor® 594

sc-390539 AF594
200 µg/ml
$357.00

JAK2 抗体 (C-10) Alexa Fluor® 647

sc-390539 AF647
200 µg/ml
$357.00

JAK2 抗体 (C-10) Alexa Fluor® 680

sc-390539 AF680
200 µg/ml
$357.00

JAK2 抗体 (C-10) Alexa Fluor® 790

sc-390539 AF790
200 µg/ml
$357.00

JAK2 抗体 (C-10) B

sc-390539 B
200 µg/ml
$320.00

JAK2 (C-10) 中和勝肽

sc-390539 P
100 µg/0.5 ml
$68.00

Hi can you provide the catalogue number for the agarose (bead) format.

Asked by: tzbenton
Thank you for your question. sc-390539 AC
Answered by: BlakeJ
Date published: 2025-04-04

What is the secondary antibody that can be used for western blot?

Asked by: Bavya
Thank you for your question. Here is the recommended secondary : https://www.scbt.com/p/m-igg2b-binding-protein-hrp
Answered by: BlakeJ
Date published: 2023-08-11

How is the cross activity? Will this antibody detect other Jaks? Such as Jak1, Tyk2 and Jak3? Is this product good enough to detect endogenous Jak2?

Asked by: LINDO
Thank you for your question. This mouse monoclonal antibody, JAK2 (C-10): sc-390539, is specific for JAK2 and not expected to cross-react with other JAK family members. We have published Western blot data demonstrating detection of endogenous JAK2 expression in HEL 92.1.7, K-562 and MEG-01 whole cell lysates. As well as in PDGF treated NIH/3T3 whole cell lysate.
Answered by: Technical Support
Date published: 2017-04-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with JAK2 (C-10): sc-390539 monoclonal antibody?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 5
Date published: 2017-01-10
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Rated 4 out of 5 by from JAK2I used 1:200 to do western blotting, and got clear band.
Date published: 2024-01-26
Rated 4 out of 5 by from GoodI bought it a couple weeks ago and It’s just amazing
Date published: 2022-08-20
Rated 5 out of 5 by from Research associateVery good results in western blotting, clean and strong signal.
Date published: 2018-11-29
Rated 5 out of 5 by from 功能很出色We used this antibody for WB, and could see specific bands at 128kD.this is a very good antibody than I bought from other companies. I used to tried many different samples, but its a wast of time. thanks to santa, thanks for your good products. You should try your best to provide more antibodies of high quality like this to us researchers.
Date published: 2018-05-25
Rated 3 out of 5 by from western blot做了几次实验，用于WB，HepG2 细胞裂解液，但没有得到结果（1：200）。看到其他评论说能做出条带，我们的问题出到哪儿呢？无论如何，santa 的服务还是值得肯定的，给个三星。
Date published: 2018-02-06
Rated 4 out of 5 by from WB实验JAK2 sc-390539 分子量较大，比较难做，其中转膜时间约需要2.5小时，这只抗体效果挺好！
Date published: 2018-01-02
Rated 4 out of 5 by from nice bandthe antibody was tested on rat tissue samples and worked well.
Date published: 2017-12-13
Rated 5 out of 5 by from good signalIt works really well, by 1:500 dilution, this product is suitable for WB and low background, It's a good choice.
Date published: 2017-10-18
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JAK2 抗体 (C-10) is rated 4.5 out of 5 by 28.
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