ITF Antibody (B-1): sc-398651

ITF Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects ITF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ITF Antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The intestinal trefoil factor (ITF) plays a crucial role in mucosal healing, particularly in the gastrointestinal tract, where epithelial cells secrete ITF in response to injury. ITF is characterized by a unique trefoil structure, consisting of three intrachain disulfide bonds that form a stable P-domain, allowing ITF to resist proteolytic degradation. ITF presence in the epithelial mucosal layer of the small intestine and colon, as well as in the brain and pituitary, underscores ITF importance in maintaining mucosal surface integrity and facilitating repair processes. ITF upregulation occurs in response to epithelial damage and various pathological conditions, including metaplasia and neoplasia, highlighting ITF potential as a biomarker for gastrointestinal health. ITF genes, along with pancreatic trefoil factor 2 and pS2, cluster on human chromosome 21q22.3, indicating a shared evolutionary origin and functional relationship among these trefoil factors.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ITF Antibody (B-1) References:

1. Goblet-cell-specific transcription of mouse intestinal trefoil factor gene results from collaboration of complex series of positive and negative regulatory elements.  |  Itoh, H., et al. 1999. Biochem J. 341 (Pt 2): 461-72. PMID: 10393106
2. Overexpression of intestinal trefoil factor in human colon carcinoma cells reduces cellular growth in vitro and in vivo.  |  Uchino, H., et al. 2000. Gastroenterology. 118: 60-9. PMID: 10611154
3. Augmented intestinal trefoil factor (TFF3) and loss of pS2 (TFF1) expression precedes metaplastic differentiation of gastric epithelium.  |  Taupin, D., et al. 2001. Lab Invest. 81: 397-408. PMID: 11310832
4. Intestinal trefoil factor induces decay-accelerating factor expression and enhances the protective activities against complement activation in intestinal epithelial cells.  |  Andoh, A., et al. 2001. J Immunol. 167: 3887-93. PMID: 11564806
5. Vangl1 protein acts as a downstream effector of intestinal trefoil factor (ITF)/TFF3 signaling and regulates wound healing of intestinal epithelium.  |  Kalabis, J., et al. 2006. J Biol Chem. 281: 6434-41. PMID: 16410243
6. hSP, the domain-duplicated homolog of pS2 protein, is co-expressed with pS2 in stomach but not in breast carcinoma.  |  Tomasetto, C., et al. 1990. EMBO J. 9: 407-14. PMID: 2303034
7. Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion.  |  Podolsky, DK., et al. 1993. J Biol Chem. 268: 6694-702. PMID: 8454642
8. Human intestinal trefoil factor is expressed in human hypothalamus and pituitary: evidence for a novel neuropeptide.  |  Probst, JC., et al. 1996. FASEB J. 10: 1518-23. PMID: 8940297
9. Human trefoil peptides: genomic structure in 21q22.3 and coordinated expression.  |  Gött, P., et al. 1996. Eur J Hum Genet. 4: 308-15. PMID: 9043862
10. Trefoil peptides: from structure to function.  |  Thim, L. 1997. Cell Mol Life Sci. 53: 888-903. PMID: 9447240
11. Short-chain fatty acids inhibit intestinal trefoil factor gene expression in colon cancer cells.  |  Tran, CP., et al. 1998. Am J Physiol. 275: G85-94. PMID: 9655688
12. Growth factors and the gastrointestinal tract.  |  Murphy, MS. 1998. Nutrition. 14: 771-4. PMID: 9785359

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ITF Antibody (B-1) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-ITF Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ITF Antibody (B-1) Specifications

Alternate Product Name: ITF

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 29-66

Epitope Target: ITF

Epitope Species: mouse

Product Reactivity/Detection: ITF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for ITF Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin