ISG15 Antibody (F-9): sc-166755
- ISG15 Antibody (F-9) is a mouse monoclonal IgG1 κ ISG15 antibody, cited in 110 publications, provided at 200 µg/ml
- raised against amino acids 1-150 mapping at the N-terminus of ISG15 of human origin
- ISG15 Antibody (F-9) is recommended for detection of ISG15 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ISG15 Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ISG15 Antibody (F-9) for WB and IHC(P) applications. These reagents are now offered in bundles with ISG15 Antibody (F-9) (see ordering information below).
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ISG15 Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects ISG15 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ISG15 monoclonal antibody (F-9) is raised against amino acids 1-150 mapping at the N-terminus of ISG15 of human origin. Anti-ISG15 antibody (F-9) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ISG15 (F-9) antibody is composed of two ubiquitin-like domains, a structural feature crucial for conjugating to target proteins and regulating their stability, activity, and localization, thereby modulating key immune signaling pathways. The interferon-induced 15 kDa protein (ISG15) plays a crucial role in immune response by functioning as a ubiquitin-like modifier, conjugating to target proteins such as JAK1 and MAPK3/ERK1 through a unique enzymatic pathway distinct from ubiquitin. This modification regulates various cellular processes, including protein stability and signaling pathways, which influence immune cell activation and response. ISG15 exhibits specific chemotactic activity towards neutrophils, promoting activation and release of eosinophil chemotactic factors, thereby enhancing immune response. Additionally, ISG15 (F-9) monoclonal antibody is involved in paracrine, autocrine, and endocrine signaling mechanisms, notably inducing interferon-gamma secretion by monocytes and macrophages, essential for effective immune communication. Predominantly expressed in muscle, epithelial, neuronal, and lymphoid cells, ISG15′s cytoplasmic localization underscores importance in various cellular functions and interactions within the immune system.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ISG15 Antibody (F-9) References:
- ISG15 in antiviral immunity and beyond. | Perng, YC. and Lenschow, DJ. 2018. Nat Rev Microbiol. 16: 423-439. PMID: 29769653
- ISG15, a Small Molecule with Huge Implications: Regulation of Mitochondrial Homeostasis. | Albert, M., et al. 2018. Viruses. 10: PMID: 30428561
- ISG15 Promotes ERK1 ISGylation, CD8+ T Cell Activation and Suppresses Ovarian Cancer Progression. | Yeung, TL., et al. 2018. Cancers (Basel). 10: PMID: 30469497
- ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity. | Liu, G., et al. 2021. Nat Microbiol. 6: 467-478. PMID: 33727702
- ISG15 is associated with cervical cancer development. | Tao, P., et al. 2022. Oncol Lett. 24: 380. PMID: 36238852
- ISG15: A link between innate immune signaling, DNA replication, and genome stability. | Wardlaw, CP. and Petrini, JHJ. 2023. Bioessays. 45: e2300042. PMID: 37147792
- ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma. | Xu, T., et al. 2023. J Exp Clin Cancer Res. 42: 182. PMID: 37501099
- Regulation of STING activity in DNA sensing by ISG15 modification. | Lin, C., et al. 2023. Cell Rep. 42: 113277. PMID: 37864791
- ISG15 mediates the function of extracellular vesicles in promoting ovarian cancer progression and metastasis. | Dorayappan, KDP., et al. 2024. J Extracell Biol. 3: e92. PMID: 38939897
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ISG15 Antibody (F-9) | sc-166755 | 200 µg/ml | $322.00 | |||
ISG15 Antibody (F-9): m-IgG Fc BP-HRP Bundle | sc-529040 | 200 µg Ab; 10 µg BP | $361.00 | |||
ISG15 Antibody (F-9): m-IgGκ BP-HRP Bundle | sc-521732 | 200 µg Ab, 40 µg BP | $361.00 | |||
ISG15 Antibody (F-9): m-IgG1 BP-HRP Bundle | sc-543284 | 200 µg Ab; 20 µg BP | $361.00 | |||
ISG15 Antibody (F-9) AC | sc-166755 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ISG15 Antibody (F-9) HRP | sc-166755 HRP | 200 µg/ml | $322.00 | |||
ISG15 Antibody (F-9) FITC | sc-166755 FITC | 200 µg/ml | $336.00 | |||
ISG15 Antibody (F-9) PE | sc-166755 PE | 200 µg/ml | $349.00 | |||
ISG15 Antibody (F-9) Alexa Fluor® 488 | sc-166755 AF488 | 200 µg/ml | $364.00 | |||
ISG15 Antibody (F-9) Alexa Fluor® 546 | sc-166755 AF546 | 200 µg/ml | $364.00 | |||
ISG15 Antibody (F-9) Alexa Fluor® 594 | sc-166755 AF594 | 200 µg/ml | $364.00 | |||
ISG15 Antibody (F-9) Alexa Fluor® 647 | sc-166755 AF647 | 200 µg/ml | $364.00 | |||
ISG15 Antibody (F-9) Alexa Fluor® 680 | sc-166755 AF680 | 200 µg/ml | $364.00 | |||
ISG15 Antibody (F-9) Alexa Fluor® 790 | sc-166755 AF790 | 200 µg/ml | $364.00 |