ISG15 Antibody (F-9) recommended for detection of ISG15 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

ISG15 Antibody (F-9): sc-166755

ISG15 Antibody (F-9) is rated 4.6 out of 5 by 22.
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Datasheets

    • Anti-ISG15 Antibody (F-9) is a mouse monoclonal IgG1 (kappa light chain) ISG15 antibody provided at 200 µg/ml
    • raised against amino acids 1-150 mapping at the N-terminus of ISG15 of human origin
    • Anti-ISG15 Antibody (F-9) is recommended for detection of ISG15 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-ISG15 Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ISG15 (F-9): sc-166755.

-The datasheet specifies this is "a mouse monoclonal antibody raised against amino acids 1-150" but ISG15 is 165 aa. Is there more information on the epitope (not highlighted in the protein sequence tab)? -5% milk in TBS 0.1% Tween 4 C overnighincubation?

Asked by: autumnos
Thank you for your question. Yes, this information is correct, the antibody is raised against a recombinant protein corresponding to amino acids 1-150 of ISG15 of human origin. The exact epitope recognized by this monoclonal antibody has not been determined. We do recommend blocking in 5% nonfat milk in TBST (0.05% Tween-20) for one hour at room temperature, and incubating the primary antibody at the starting dilution of 1:200 in 5% nonfat milk + TBST for one hour at room temperature, or overnight at 4C.
Answered by: Technical Support
Date published: 2019-07-03

What is the light chain of ISG15 Antibody (F-9): sc-166755 ?

Asked by: cjMara
ISG15 Antibody (F-9) is a mouse monoclonal IgG1 with kappa light chain.
Answered by: Technical Support 9
Date published: 2017-09-13
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Rated 5 out of 5 by from The antibody is good I got a free test antibody, the antibody is very good, it also can be used for IP.
Date published: 2019-02-26
Rated 5 out of 5 by from Strong signal F-9 antibody produces a strong signal on WB at 1:1000 dilution. The band observed is a bit "lighter" than expected but that's probably caused by using Tris-Glycine system.
Date published: 2018-02-02
Rated 5 out of 5 by from sc-166755 This antibody works great for WB (cell lysates; mouse)
Date published: 2018-01-02
Rated 5 out of 5 by from Nice IHC staining I used it for IHC of human cells an gives an intense and specific signal.
Date published: 2017-12-22
Rated 5 out of 5 by from Works good in WB This Ab with 1:1000 dilution was used for the detection of human samples and the interested band could be observed clearly in WB image.
Date published: 2017-09-28
Rated 5 out of 5 by from an excellent antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 1 out of 5 by from this antibody did not work by WB of A549 cell (1:1 this antibody did not work by WB of A549 cell (1:1000 overnight)
Date published: 2017-09-15
Rated 5 out of 5 by from Good antibody. It works well for WB (dilution 1:500) and IF (dilution 1:100).
Date published: 2017-08-14
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