ISG15 Antibody (A-4): sc-514964

ISG15 Antibody (A-4) is a mouse monoclonal IgG2a antibody that detects ISG15 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ISG15 antibody (A-4) is specific for an epitope mapping between amino acids 52-73 within an internal region of ISG15 of human origin. ISG15, a 15 kDa protein, functions as a ubiquitin-like modifier, playing a crucial role in the post-translational modification of various intracellular target proteins, including JAK1 and MAPK3/ERK1. This modification is essential for regulating diverse cellular processes, such as immune response and inflammation, by influencing protein stability and activity. ISG15 (A-4) antibody has been implicated in the pathogenesis of viral infections, autoimmune diseases such as systemic lupus erythematosus, and cancer, making ISG15 a critical target for experimental studies exploring therapeutic interventions in these conditions. Anti-ISG15 antibody (A-4) conjugates to target proteins through a distinct enzymatic pathway from ubiquitin, highlighting ISG15′s unique role in cellular signaling. Additionally, ISG15 (A-4) monoclonal antibody exhibits specific chemotactic activity towards neutrophils, activating them to release eosinophil chemotactic factors, which further underscores ISG15′s importance in immune modulation. Predominantly expressed in muscle, epithelial, neuronal, and lymphoid cells, ISG15 is localized in the cytoplasm, where ISG15 participates in paracrine, autocrine, and endocrine signaling mechanisms, including the induction of interferon-gamma secretion by monocytes and macrophages. ISG15 (A-4) monoclonal antibody serves as an invaluable tool for researchers studying the intricate roles of ISG15 in immune responses and cellular signaling pathways.

The preferred ISG15 antibody is ISG15 Antibody (3E5): sc-69701.