IRS-1 Antibody (H-7): sc-515017

IRS-1 Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects IRS-1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IRS-1 (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The insulin receptor substrate-1 (IRS-1) plays a crucial role in insulin signaling as a major substrate of the insulin receptor, being phosphorylated in response to insulin, insulin-like growth factor 1 (IGF-1), and interleukin 4 (IL-4) stimulation. Phosphorylation occurs on serine, threonine, and tyrosine residues across various tissues, with casein kinase II being a key serine/threonine kinase that mediates a significant portion of insulin-stimulated phosphorylation of IRS-1. Notably, threonine 502 has been identified as the primary phosphorylation site for casein kinase II in rat IRS-1, while serine 99 serves as an additional phosphorylation site. Phosphorylation of IRS-1 by casein kinase II is an integral part of the intracellular insulin signaling cascade, influencing metabolic responses to insulin. Furthermore, IRS-1 contains three putative binding sites for the 14-3-3 protein, which are critical for insulin receptor interaction. Binding of 14-3-3 to IRS-1 is significantly enhanced following treatment with okadaic acid, a potent inhibitor of serine/threonine phosphatases, suggesting that 14-3-3 may modulate insulin sensitivity by disrupting IRS-1 and insulin receptor interaction.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IRS-1 Antibody (H-7) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-IRS-1 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IRS-1 Antibody (H-7) Specifications

Alternate Product Name: IRS-1

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-25

Epitope Target: IRS-1

Epitope Species: human

Product Reactivity/Detection: IRS-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for IRS-1 Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred IRS-1 antibody is IRS-1 Antibody (E-12): sc-8038.