IRS-1 Antibody (E-12): sc-8038

IRS-1 Antibody (E-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects IRS-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). IRS-1 Antibody (E-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The insulin receptor substrate-1 (IRS-1) plays a crucial role in insulin signaling as a major substrate of the insulin receptor, facilitating the transduction of signals that regulate glucose homeostasis and cellular metabolism. Located primarily in the cytoplasm, IRS-1 is essential for mediating the effects of insulin, insulin-like growth factor 1 (IGF-1), and interleukin 4 (IL-4) by undergoing phosphorylation on serine, threonine, and tyrosine residues in response to these stimuli. Notably, IRS-1 contains three putative binding sites for the 14-3-3 protein, which are critical for its interaction with the insulin receptor and may influence insulin sensitivity. The phosphorylation of IRS-1 by casein kinase II, particularly at Thr 502 and Ser 99, is significant as it may modulate the intracellular insulin signaling cascade, highlighting the importance of post-translational modifications in regulating IRS-1 function and its downstream effects on metabolic pathways.

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IRS-1 Antibody (E-12) References:

1. IRS-1 activates phosphatidylinositol 3'-kinase by associating with src homology 2 domains of p85.  |  Myers, MG., et al. 1992. Proc Natl Acad Sci U S A. 89: 10350-4. PMID: 1332046
2. Divergent roles for IRS-1 and IRS-2 in breast cancer metastasis.  |  Gibson, SL., et al. 2007. Cell Cycle. 6: 631-7. PMID: 17361103
3. Nuclear IRS-1 and cancer.  |  Reiss, K., et al. 2012. J Cell Physiol. 227: 2992-3000. PMID: 22454254
4. Insulin receptor substrate-1 (IRS-1) forms a ribonucleoprotein complex associated with polysomes.  |  Ozoe, A., et al. 2013. FEBS Lett. 587: 2319-24. PMID: 23770097
5. Distinct signalling properties of insulin receptor substrate (IRS)-1 and IRS-2 in mediating insulin/IGF-1 action.  |  Rabiee, A., et al. 2018. Cell Signal. 47: 1-15. PMID: 29550500
6. Insulin receptor substrate-1 (IRS-1) mediates progesterone receptor-driven stemness and endocrine resistance in oestrogen receptor+ breast cancer.  |  Dwyer, AR., et al. 2021. Br J Cancer. 124: 217-227. PMID: 33144693
7. VAPB-mediated ER-targeting stabilizes IRS-1 signalosomes to regulate insulin/IGF signaling.  |  Gao, XK., et al. 2023. Cell Discov. 9: 83. PMID: 37528084
8. Phosphorylation Codes in IRS-1 and IRS-2 Are Associated with the Activation/Inhibition of Insulin Canonical Signaling Pathways.  |  Martínez Báez, A., et al. 2024. Curr Issues Mol Biol. 46: 634-649. PMID: 38248343
9. Phosphorylation of the insulin receptor substrate IRS-1 by casein kinase II.  |  Tanasijevic, MJ., et al. 1993. J Biol Chem. 268: 18157-66. PMID: 8349691
10. IRS-1 is a common element in insulin and insulin-like growth factor-I signaling to the phosphatidylinositol 3'-kinase.  |  Myers, MG., et al. 1993. Endocrinology. 132: 1421-30. PMID: 8384986
11. The new elements of insulin signaling. Insulin receptor substrate-1 and proteins with SH2 domains.  |  Myers, MG. and White, MF. 1993. Diabetes. 42: 643-50. PMID: 8387037
12. 14-3-3 protein binds to insulin receptor substrate-1, one of the binding sites of which is in the phosphotyrosine binding domain.  |  Ogihara, T., et al. 1997. J Biol Chem. 272: 25267-74. PMID: 9312143

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IRS-1 Antibody (E-12) has 78 citations in a variety of scientific publications.

Primary antibodies like Anti-IRS-1 Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IRS-1 Antibody (E-12) Specifications

Alternate Product Name: IRS-1

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 905-1070

Epitope Target: IRS-1

Epitope Species: human

Product Reactivity/Detection: IRS-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 78 citations in various scientific publications

Available Conjugates/Forms for IRS-1 Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred IRS-1 antibody is IRS-1 Antibody (H-7): sc-515017.