IRP-1 Antibody (E-12): sc-166022

IRP-1 Antibody (E-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects IRP-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IRP-1 (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IRP-1 plays a crucial role in iron metabolism, which is vital for maintaining cellular homeostasis. IRP-1 functions as a regulatory factor that modulates iron uptake and storage in response to cellular iron levels and stress signals. Specifically, IRP-1 can bind to iron-responsive elements (IREs) in mRNA, thereby influencing the translation of proteins involved in iron transport and storage. This regulatory mechanism is essential because dysregulation of iron homeostasis can lead to various diseases, including anemia and neurodegenerative disorders. Using anti-IRP-1 antibody (E-12) in research can provide insights into the intricate balance of iron metabolism and its implications in health and disease.

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IRP-1 Antibody (E-12) References:

1. Effects of 12 metal ions on iron regulatory protein 1 (IRP-1) and hypoxia-inducible factor-1 alpha (HIF-1alpha) and HIF-regulated genes.  |  Li, Q., et al. 2006. Toxicol Appl Pharmacol. 213: 245-55. PMID: 16386771
2. Expression of active iron regulatory factor from a full-length human cDNA by in vitro transcription/translation.  |  Hirling, H., et al. 1992. Nucleic Acids Res. 20: 33-9. PMID: 1738601
3. Homology between IRE-BP, a regulatory RNA-binding protein, aconitase, and isopropylmalate isomerase.  |  Hentze, MW. and Argos, P. 1991. Nucleic Acids Res. 19: 1739-40. PMID: 1903202
4. A regulated RNA binding protein also possesses aconitase activity.  |  Kaptain, S., et al. 1991. Proc Natl Acad Sci U S A. 88: 10109-13. PMID: 1946430
5. Interaction of iron regulatory protein-1 (IRP-1) with ATP/ADP maintains a non-IRE-binding state.  |  Popovic, Z. and Templeton, DM. 2010. Biochem J. 430: 315-24. PMID: 20569198
6. Cloning of the cDNA encoding an RNA regulatory protein--the human iron-responsive element-binding protein.  |  Rouault, TA., et al. 1990. Proc Natl Acad Sci U S A. 87: 7958-62. PMID: 2172968
7. Lead induces dysregulation of iron regulatory protein 1 via the extracellular signal-regulated kinase pathway in human vascular endothelial cells.  |  Wang, Q., et al. 2012. Brain Res. 1455: 19-27. PMID: 22502979
8. Nucleotide-specific recognition of iron-responsive elements by iron regulatory protein 1.  |  Selezneva, AI., et al. 2013. J Mol Biol. 425: 3301-10. PMID: 23806658
9. A synergistic role of IRP1 and FBXL5 proteins in coordinating iron metabolism during cell proliferation.  |  Johnson, NB., et al. 2017. J Biol Chem. 292: 15976-15989. PMID: 28768766
10. Iron regulatory protein 1 promotes ferroptosis by sustaining cellular iron homeostasis in melanoma.  |  Yao, F., et al. 2021. Oncol Lett. 22: 657. PMID: 34386079
11. The impact of oxidative stress on eukaryotic iron metabolism.  |  Rouault, TA. and Klausner, RD. 1996. EXS. 77: 183-97. PMID: 8856975
12. Abnormal localization of iron regulatory protein in Alzheimer's disease.  |  Smith, MA., et al. 1998. Brain Res. 788: 232-6. PMID: 9555030

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IRP-1 Antibody (E-12) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-IRP-1 Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IRP-1 Antibody (E-12) Specifications

Alternate Product Name: IRP-1

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 3-41

Epitope Target: IRP-1

Epitope Species: human

Product Reactivity/Detection: IRP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for IRP-1 Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin