IRP-1 Antibody (E-12) recommended for detection of IRP-1 of mouse, rat and human origin by WB, IP, IF and ELISA

IRP-1 Antibody (E-12): sc-166022

IRP-1 Antibody (E-12) is rated 4.8 out of 5 by 5.
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Datasheets

    • Anti-IRP-1 Antibody (E-12) is a mouse monoclonal IgG2a (kappa light chain) IRP-1 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 3-41 at the N-terminus of IRP-1 of human origin
    • Anti-IRP-1 Antibody (E-12) is recommended for detection of IRP-1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-IRP-1 Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-166022 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IRP-1 (E-12): sc-166022.

I want to know whether this antibody can be used to detect endogenous IRP1 in cell for WB experiment

Asked by: Anonymous
Thank you for your question. Yes, this antibody can detect endogenous IFP1 via Western blot in mouse, rat and human samples.
Answered by: Tech Service
Date published: 2019-12-02

What application is the blocking peptide sc-166022 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from pretty good antibody It was used for western blot and we used N2A cells. It worked pretty good, the bands were clearly obtained.
Date published: 2019-04-01
Rated 5 out of 5 by from Good antibody I can get a clear band above 100kd without nonspecific band
Date published: 2017-08-01
Rated 5 out of 5 by from Very Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-26
Rated 5 out of 5 by from Published western blot data with HL60 human Published western blot data with HL60 human myelocytic leukemia cell lysates. -SCBT Publication Review
Date published: 2015-02-01
Rated 5 out of 5 by from Clean bands in western blot using human IRP Clean bands in western blot using human IRP-1 transfected lysates. -SCBT QC
Date published: 2014-05-03
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