IRF-7 Antibody (F-1) recommended for detection of IRF-7 of mouse, rat and human origin by WB, IP, IF and ELISA

IRF-7 Antibody (F-1): sc-74471

IRF-7 Antibody (F-1) is rated 4.4 out of 5 by 7.
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Datasheets

    • IRF-7 Antibody (F-1) is a mouse monoclonal IgG2a κ, cited in 14 publications, provided at 200 µg/ml
    • raised against amino acids 1-246 of IRF-7 of human origin
    • Anti-IRF-7 Antibody (F-1) is recommended for detection of IRF-7 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-IRF-7 Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-74471 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IRF-7 (F-1): sc-74471.

I would like to know if it antibody works very well to detect IRF7 in mouse astrocytes. It is possible to receive a free sample to test it? Thanks a lot,

Asked by: Paola13
Thank you for your question. We have not specifically tested this antibody in astrocytes, however the antibody is confirmed to be reactive with mouse IRF-7. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-04-24

What is the recommended secondary reagent to use with IRF-7 (F-1): sc-74471 monoclonal antibody for Western blot?

Asked by: cjMara
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. This primary antibody has a kappa light chain, so for Western blotting we would recommend m-IgGκ BP-HRP: sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 5
Date published: 2017-01-20
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Rated 5 out of 5 by from Works well in WB. Works well with fibroblast lysate in 1:200 dilution.
Date published: 2018-05-17
Rated 5 out of 5 by from Good signal on western blot Works well on western blot. Clear bands between 55kD and 72kD.
Date published: 2017-12-11
Rated 5 out of 5 by from Strong WB Great product for western blot applications; strong signal
Date published: 2017-02-05
Rated 5 out of 5 by from Good alternative to polyclonal The F-1 clone is a good alternative to the now discontinued polyclonal, IRF-7 (H-246): sc-9083. It is raised against the same epitope and has been featured in several publications.
Date published: 2016-12-29
Rated 2 out of 5 by from Detecting the IRF Detecting the IRF-7 on hela cells which is infected by virus but the band is not strong.
Date published: 2015-10-29
Rated 4 out of 5 by from Single band at correct mw with little background Single band at correct mw with little background in Jurkat, PMA-treated Jurkat and Raji whole cell lysates.
Date published: 2013-07-25
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