IRF-7 Antibody (G-8) recommended for detection of IRF-7 of human origin by WB, IP, IF, IHC(P) and ELISA

IRF-7 Antibody (G-8): sc-74472

IRF-7 Antibody (G-8) is rated 4.7 out of 5 by 10.
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Datasheets

    • Anti-IRF-7 Antibody (G-8) is a mouse monoclonal IgG2a κ IRF-7 antibody, cited in 24 publications, provided at 200 µg/ml
    • raised against amino acids 1-246 of IRF-7 of human origin
    • IRF-7 Antibody (G-8) is recommended for detection of IRF-7 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-IRF-7 Antibody (G-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-74472 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IRF-7 (G-8): sc-74472.

Hi, Please do you know whether this antibody works fine for the detection of Equine IRF-7 protein using WB? Thank you

Asked by: Fatai
Thank you for your question. We have not tested this antibody, IRF-7 (G-8): sc-74472, in equine samples, and do not know if it will work for that species. The antibody is raised against an antigen corresponding to amino acids 1-246 of IRF-7 of human origin, which shares ~65% sequence identity with a predicted protein sequence for interferon regulatory factor 7-like in Equus caballus (protein accession number: XP_001494207). This is below our typical threshold for recommended cross-reactivity in another species, but it is significant and the antibody may have an epitope that would work for equine. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to request a free sample to test in your species. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-08-21

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-17
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Rated 5 out of 5 by from Works well in WB. Works well with fibroblast lysate in 1:200 dilution.
Date published: 2018-05-17
Rated 4 out of 5 by from Bands in HEK293 cells Clear bands in HEK293 cell lysates using 20ug of protein (dilution 1:250)
Date published: 2018-02-20
Rated 5 out of 5 by from Good antibody I used this antibody and nonspecific band were detected! It's a good one.
Date published: 2017-03-16
Rated 5 out of 5 by from Great IRF-7 antibody IRF-7 (G-8) gave beautiful staining of HeLa cells for Immunofluorescence (IF).
Date published: 2017-01-23
Rated 5 out of 5 by from Great antibody Strong antibody for both western blot and IF staining
Date published: 2017-01-23
Rated 4 out of 5 by from good Worked well in WB in human 293T whole cell lysates, Strong band in WB
Date published: 2016-10-28
Rated 5 out of 5 by from Performed well in IF with methanol Performed well in IF with methanol-fixed HeLa cells showed cytoplasmic and nuclear localization. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Worked well with formalin fixed Worked well with formalin fixed, paraffin-embedded human fallopian tube tissue showed nuclear staining of glandular cells at low and high magnification. -SCBT QC
Date published: 2015-01-05
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