IRAK-1 Antibody (B-5): sc-55530

IRAK-1 Antibody (B-5) is a mouse monoclonal IgG2b antibody that detects IRAK-1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IRAK-1, or IL-1 receptor-associated kinase 1, plays a crucial role in the signaling pathways activated by interleukin-1 (IL-1), particularly in the activation of the NF-kB pathway, which is essential for regulating immune responses and inflammation. Located primarily in the cytoplasm, IRAK-1 is recruited to the IL-1 receptor complex upon ligand binding, where IRAK-1 undergoes phosphorylation and activation. This process is vital as IRAK-1 leads to the downstream activation of various transcription factors, including NF-kB, which in turn regulates the expression of numerous pro-inflammatory cytokines. The proper functioning of IRAK-1 is critical for maintaining immune homeostasis, and dysregulation of IRAK-1 activity has been implicated in various inflammatory diseases and conditions, making IRAK-1 a significant target for therapeutic intervention. Anti-IRAK-1 antibody (B-5) is an invaluable tool for researchers studying the intricate mechanisms of immune signaling and the pathophysiology of related diseases.

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IRAK-1 Antibody (B-5) References:

1. Translocation of the interleukin-1 receptor-associated kinase-1 (IRAK-1) into the nucleus.  |  Böl, G., et al. 2000. FEBS Lett. 477: 73-8. PMID: 10899313
2. The death domain of IRAK-1: an oligomerization domain mediating interactions with MyD88, Tollip, IRAK-1, and IRAK-4.  |  Neumann, D., et al. 2007. Biochem Biophys Res Commun. 354: 1089-94. PMID: 17276401
3. Leishmania-induced IRAK-1 inactivation is mediated by SHP-1 interacting with an evolutionarily conserved KTIM motif.  |  Abu-Dayyeh, I., et al. 2008. PLoS Negl Trop Dis. 2: e305. PMID: 19104650
4. Regulation of IRAK-1 activation by its C-terminal domain.  |  Nguyen, T., et al. 2009. Cell Signal. 21: 719-26. PMID: 19166926
5. Gender dependent importance of IRAK-1 in dextran sulfate sodium induced colitis.  |  Berglund, M., et al. 2009. Cell Immunol. 259: 27-32. PMID: 19540456
6. IRAK-1 contributes to lipopolysaccharide-induced reactive oxygen species generation in macrophages by inducing NOX-1 transcription and Rac1 activation and suppressing the expression of antioxidative enzymes.  |  Maitra, U., et al. 2009. J Biol Chem. 284: 35403-11. PMID: 19850916
7. Interleukin-1 Receptor-Associated Kinase-1 (IRAK-1) functionally associates with PKCepsilon and VASP in the regulation of macrophage migration.  |  Gan, L. and Li, L. 2010. Mol Immunol. 47: 1278-82. PMID: 20044140
8. Differential regulation of interleukin-1 receptor-associated kinase-1 (IRAK-1) and IRAK-2 by microRNA-146a and NF-kappaB in stressed human astroglial cells and in Alzheimer disease.  |  Cui, JG., et al. 2010. J Biol Chem. 285: 38951-60. PMID: 20937840
9. Increased expression of the interleukin-1 receptor-associated kinase (IRAK)-1 is associated with adipose tissue inflammatory state in obesity.  |  Ahmad, R., et al. 2015. Diabetol Metab Syndr. 7: 71. PMID: 26312071
10. Inherited human IRAK-1 deficiency selectively impairs TLR signaling in fibroblasts.  |  Della Mina, E., et al. 2017. Proc Natl Acad Sci U S A. 114: E514-E523. PMID: 28069966

The preferred IRAK-1 antibody is IRAK-1 Antibody (F-4): sc-5288.