Integrin α6/ITGA6/CD49f Antibody (3H1512): sc-71423

Integrin α6 Antibody (3H1512) is a mouse monoclonal IgG1 kappa light chain antibody raised against integrin α6/β4 from A-431 cells of human origin. Anti-integrin α6 antibody (3H1512) specifically recognizes integrin α6, also known as CD49f or ITGA6, in human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Integrin α6 is a transmembrane glycoprotein that pairs with β4 or β1 subunits to form heterodimeric receptors like α6β4 and α6β1, which are essential for binding to laminin in the extracellular matrix. Structurally, integrin α6 consists of a large extracellular domain for ligand binding, a single transmembrane domain, and a short cytoplasmic tail that interacts with intracellular signaling molecules and adaptor proteins, such as talin and vinculin, linking integrin α6 to the actin cytoskeleton. This structural configuration enables integrin α6 to mediate cell adhesion, migration, and signal transduction, playing a vital role in maintaining tissue integrity and cellular communication. Alterations in the structure or expression of integrin α6, particularly in epithelial-to-mesenchymal transition (EMT), can significantly impact biological processes such as wound healing, embryonic development, and cancer progression. Integrin α6 monoclonal antibody (3H1512) serves as a valuable tool for researchers studying cell adhesion, migration, and related signaling pathways in developmental biology, oncology, and other fields.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Integrin α6/ITGA6/CD49f Antibody (3H1512) References:

1. Thrombospondin-1 (TSP-1) Stimulates Expression of Integrin alpha6 in Human Breast Carcinoma Cells: A Downstream Modulator of TSP-1-Induced Cellular Adhesion.  |  John, AS., et al. 2010. J Oncol. 2010: 645376. PMID: 20631908
2. Site-specific S-nitrosylation of integrin α6 increases the extent of prostate cancer cell migration by enhancing integrin β1 association and weakening adherence to laminin-1.  |  Isaac, J., et al. 2012. Biochemistry. 51: 9689-97. PMID: 23106339
3. Fn14 receptor promotes invasive potential and metastatic capacity of non-small lung adenocarcinoma cells through the up-regulation of integrin α6.  |  Jandova, J., et al. 2015. Neoplasma. 62: 41-52. PMID: 25563366
4. Hair-growth-promoting effect of conditioned medium of high integrin α6 and low CD 71 (α6bri/CD71dim) positive keratinocyte cells.  |  Won, CH., et al. 2015. Int J Mol Sci. 16: 4379-91. PMID: 25706512
5. The Role of Integrin α6 (CD49f) in Stem Cells: More than a Conserved Biomarker.  |  Krebsbach, PH. and Villa-Diaz, LG. 2017. Stem Cells Dev. 26: 1090-1099. PMID: 28494695
6. Upregulation of Integrin-α6 and Integrin-β1 Gene Expressions in Mouse Spermatogonial Stem Cells after Continues and Pulsed Low Intensity Ultrasound Stimulation.  |  Mohaqiq, M., et al. 2018. Cell J. 19: 634-639. PMID: 29105399
7. Integrin α6 (CD49f), The Microenvironment and Cancer Stem Cells.  |  Bigoni-Ordóñez, GD., et al. 2019. Curr Stem Cell Res Ther. 14: 428-436. PMID: 30280675
8. The calcium channel TRPC6 promotes chemotherapy-induced persistence by regulating integrin α6 mRNA splicing.  |  Mukhopadhyay, D., et al. 2023. Cell Rep. 42: 113347. PMID: 37910503
9. Abrogation of lung metastasis of human fibrosarcoma cells by ribozyme-mediated suppression of integrin alpha6 subunit expression.  |  Yamamoto, H., et al. 1996. Int J Cancer. 65: 519-24. PMID: 8621237
10. A novel homozygous mutation affecting integrin alpha6 in a case of junctional epidermolysis bullosa with pyloric atresia detected in utero by ultrasound examination.  |  Gache, Y., et al. 1998. J Invest Dermatol. 111: 914-6. PMID: 9804362

The preferred Integrin α6/ITGA6/CD49f antibody is Integrin α6/ITGA6/CD49f Antibody (GOH3): sc-19622.