Integrin α5/ITGA5/CD49e Antibody (A-11): sc-166665

Integrin α5 Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Integrin α5 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Integrin α5 antibody (A-11) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Integrin α5 is a crucial component of the integrin family, which consists of heterodimers formed by noncovalently associated transmembrane α and β subunits. Integrin α5 plays a significant role in mediating cell adhesion to the extracellular matrix, specifically binding to ligands such as fibronectin, collagen, and vitronectin. This interaction is vital for various cellular processes, including cell migration, proliferation, and differentiation, as it facilitates the transmission of signals from the extracellular environment to the cell interior. Integrin α5 is involved in the formation of focal adhesions, which are critical for maintaining cell shape and stability, and for orchestrating cellular responses to mechanical stimuli. Integrin α5 (A-11) monoclonal antibody enables researchers to gain valuable insights into the mechanisms of cell adhesion and signaling, which are fundamental to understanding various physiological and pathological processes.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

Download thiomersal SDS

Integrin α5/ITGA5/CD49e Antibody (A-11) References:

1. Integrin alpha5/beta1 mediates fibronectin-dependent epithelial cell proliferation through epidermal growth factor receptor activation.  |  Kuwada, SK. and Li, X. 2000. Mol Biol Cell. 11: 2485-96. PMID: 10888683
2. The PDZ domain of TIP-2/GIPC interacts with the C-terminus of the integrin alpha5 and alpha6 subunits.  |  El Mourabit, H., et al. 2002. Matrix Biol. 21: 207-14. PMID: 11852236
3. Integrin alpha5 is involved in fibronectin-induced human extravillous trophoblast invasion.  |  Zeng, BX., et al. 2007. J Reprod Immunol. 73: 1-10. PMID: 17027088
4. ZEB2 upregulates integrin α5 expression through cooperation with Sp1 to induce invasion during epithelial-mesenchymal transition of human cancer cells.  |  Nam, EH., et al. 2012. Carcinogenesis. 33: 563-71. PMID: 22227038
5. Twist1 and AP-1 cooperatively upregulate integrin α5 expression to induce invasion and the epithelial-mesenchymal transition.  |  Nam, EH., et al. 2015. Carcinogenesis. 36: 327-37. PMID: 25600770
6. Activation of integrin α5 mediated by flow requires its translocation to membrane lipid rafts in vascular endothelial cells.  |  Sun, X., et al. 2016. Proc Natl Acad Sci U S A. 113: 769-74. PMID: 26733684
7. RUNX2 promotes breast cancer bone metastasis by increasing integrin α5-mediated colonization.  |  Li, XQ., et al. 2016. Cancer Lett. 380: 78-86. PMID: 27317874
8. Clustering of integrin α5 at the lateral membrane restores epithelial polarity in invasive colorectal cancer cells.  |  Starchenko, A., et al. 2017. Mol Biol Cell. 28: 1288-1300. PMID: 28356422
9. CircRNA-vgll3 promotes osteogenic differentiation of adipose-derived mesenchymal stem cells via modulating miRNA-dependent integrin α5 expression.  |  Zhang, D., et al. 2021. Cell Death Differ. 28: 283-302. PMID: 32814879
10. Anticancer and chemosensitization effects of cannabidiol in 2D and 3D cultures of TNBC: involvement of GADD45α, integrin-α5, -β5, -β1, and autophagy.  |  Surapaneni, SK., et al. 2022. Drug Deliv Transl Res. 12: 2762-2777. PMID: 35217991

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Integrin α5/ITGA5/CD49e Antibody (A-11) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Integrin α5/ITGA5/CD49e Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Integrin α5/ITGA5/CD49e Antibody (A-11) Specifications

Alternate Product Name: Integrin α5

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 840-943

Epitope Target: Integrin α5

Epitope Species: human

Product Reactivity/Detection: Integrin α5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Integrin α5/ITGA5/CD49e Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Integrin α5/ITGA5/CD49e antibody is Integrin α5/ITGA5/CD49e Antibody (P1D6): sc-13547.