Int-6 Antibody (D-8): sc-376899
- Int-6 Antibody (D-8) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 415-444 at the C-terminus of Int-6 of mouse origin
- recommended for detection of Int-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and porcine
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Int-6 Antibody (D-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Int-6 Antibody (D-8) (see ordering information below).
QUICK LINKS
SEE ALSO...
Int-6 Antibody (D-8) is a mouse monoclonal IgG2a antibody that detects Int-6 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Int-6, also known as eIF3e or eIF3-p46, plays a crucial role in regulating translation and protein degradation by interacting with multiple complexes, including the eukaryotic translation initiation factor 3 (eIF3), the proteasome regulatory lid, and the constitutive photomorphogenesis 9 signalosome. Int-6′s involvement in the assembly of 40S ribosomal subunits on mRNA is vital for initiating protein synthesis, which is essential for cell growth and function. Notably, Int-6 is a significant site of integration for the mouse mammary tumor virus (MMTV) in murine tumors, highlighting Int-6′s potential role in cancer biology. Furthermore, studies have shown that reducing Int-6 expression in HeLa cells can lead to defects in mitosis, including issues with spindle formation, chromosome segregation, and cytokinesis, which are associated with the inhibition of cyclin B-Cdk1 kinase activity due to a prolonged inhibitory phosphorylated state of Cdk1. This underscores Int-6′s importance in maintaining proper cell cycle progression and potential implications in tumorigenesis.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Int-6 Antibody (D-8) | sc-376899 | 200 µg/ml | $322.00 | |||
Int-6 Antibody (D-8): m-IgG Fc BP-HRP Bundle | sc-538008 | 200 µg Ab; 10 µg BP | $361.00 | |||
Int-6 Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-535523 | 200 µg Ab; 40 µg BP | $361.00 | |||
Int-6 Antibody (D-8): m-IgG2a BP-HRP Bundle | sc-548182 | 200 µg Ab; 10 µg BP | $361.00 | |||
Int-6 (D-8) Neutralizing Peptide | sc-376899 P | 100 µg/0.5 ml | $69.00 |