Insulin Antibody (4H8): sc-52037
- Insulin Antibody (4H8) is a mouse monoclonal IgG1 provided at 100 µg/ml
- raised against human recombinant C-peptide construction
- recommended for detection of free C-peptide and C-peptide region in proinsulin molecules of human origin by WB, IP and ELISA; also reactive with additional species, including and bovine and porcine
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Insulin Antibody (4H8). This work is in progress.
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Insulin Antibody (4H8) is a mouse monoclonal IgG1 antibody designed to target the human recombinant C-peptide of insulin, offering exceptional precision for applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Insulin monoclonal antibody (4H8) not only exhibits strong reactivity with human insulin but also cross-reacts with insulin from additional species, including bovine and porcine, providing versatile utility for a wide range of comparative studies across different biological systems. Insulin is a vital peptide hormone synthesized and secreted by the pancreatic beta cells, playing a crucial role in regulating glucose metabolism by facilitating the uptake of glucose into cells, promoting glycogen synthesis, and thereby lowering blood glucose levels. Within the pancreatic beta cells, insulin is initially produced as preproinsulin in the rough endoplasmic reticulum, which is then processed in the Golgi apparatus to form proinsulin. Proinsulin is subsequently cleaved to generate the mature insulin hormone, consisting of an A chain and a B chain connected by disulfide bridges, and a C-peptide that is released during this maturation process. The precise localization of insulin within secretory granules in the beta cells is essential for regulated secretion in response to elevated blood glucose levels, ensuring timely and appropriate metabolic responses. Insulin exerts effects by binding to insulin receptors on the surface of target cells, initiating intracellular signaling pathways that enhance glucose uptake, increase cell permeability to monosaccharides, amino acids, and fatty acids, and regulate various aspects of cellular metabolism and growth. Dysregulation of insulin synthesis, processing, or signaling can lead to significant metabolic disorders such as hyperproinsulinemia and type 2 diabetes mellitus, highlighting the importance of insulin′s cellular location and function in maintaining metabolic homeostasis. Insulin (4H8) antibody serves as an indispensable tool for researchers studying insulin signaling pathways, metabolic regulation, and the pathophysiology of diabetes, thereby facilitating advancements in understanding and treatment of these critical health conditions.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Insulin Antibody (4H8) | sc-52037 | 100 µg/ml | $316.00 |