INSM1 Antibody (A-8): sc-271408

INSM1 Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects INSM1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-INSM1 antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. INSM1, also known as insulinoma-associated protein 1 or zinc finger protein IA-1, is a crucial developmentally regulated zinc finger transcription factor that plays a significant role in neuroendocrine differentiation. INSM1 is primarily localized in the nucleus and is expressed in embryonic tissues, particularly during the differentiation of neuroendocrine cells, while being absent in normal adult tissues. Notably, INSM1 is highly expressed in neuroendocrine tumors, making INSM1 a potential biomarker for these malignancies. INSM1 features five Cys(2)-His(2)-type zinc finger DNA binding domains and a prohormone domain, which are essential for function as a transcriptional repressor of the Neuro D promoter. By recruiting cyclin D1 as a co-repressor, INSM1 is vital for the normal differentiation of pancreatic endocrine cells, and INSM1 inhibition leads to a decrease in the formation of glucagon and insulin-positive cells. The gene encoding INSM1 is directly regulated by Neurogenin 3, which binds to the chromatin in the INSM1 promoter region to induce transcription, highlighting the intricate regulatory mechanisms governing neuroendocrine development.

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INSM1 Antibody (A-8) References:

1. Neuroendocrine differentiation factor, IA-1, is a transcriptional repressor and contains a specific DNA-binding domain: identification of consensus IA-1 binding sequence.  |  Breslin, MB., et al. 2002. Nucleic Acids Res. 30: 1038-45. PMID: 11842116
2. The zinc-finger transcription factor INSM1 is expressed during embryo development and interacts with the Cbl-associated protein.  |  Xie, J., et al. 2002. Genomics. 80: 54-61. PMID: 12079283
3. NeuroD1/E47 regulates the E-box element of a novel zinc finger transcription factor, IA-1, in developing nervous system.  |  Breslin, MB., et al. 2003. J Biol Chem. 278: 38991-7. PMID: 12890672
4. Expression of two insm1-like genes in the developing zebrafish nervous system.  |  Lukowski, CM., et al. 2006. Gene Expr Patterns. 6: 711-8. PMID: 16487754
5. IA1 is NGN3-dependent and essential for differentiation of the endocrine pancreas.  |  Mellitzer, G., et al. 2006. EMBO J. 25: 1344-52. PMID: 16511571
6. INSM1 functions as a transcriptional repressor of the neuroD/beta2 gene through the recruitment of cyclin D1 and histone deacetylases.  |  Liu, WD., et al. 2006. Biochem J. 397: 169-77. PMID: 16569215
7. The zinc-finger factor Insm1 (IA-1) is essential for the development of pancreatic beta cells and intestinal endocrine cells.  |  Gierl, MS., et al. 2006. Genes Dev. 20: 2465-78. PMID: 16951258
8. Neurogenin 3 recruits CBP co-activator to facilitate histone H3/H4 acetylation in the target gene INSM1.  |  Breslin, MB., et al. 2007. FEBS Lett. 581: 949-54. PMID: 17300785
9. Epistatic genetic interactions between Insm1 and Ikzf2 during cochlear outer hair cell development.  |  Li, S., et al. 2023. Cell Rep. 42: 112504. PMID: 37171961
10. Small Molecules Targeting INSM1 for the Treatment of High-Risk Neuroblastoma.  |  Lan, MS. and Chen, C. 2023. Biology (Basel). 12: PMID: 37627018
11. Insm1 regulates mTEC development and immune tolerance.  |  Tao, W., et al. 2023. Cell Mol Immunol. 20: 1472-1486. PMID: 37990032
12. Molecular characterization of the promoter region of a neuroendocrine tumor marker, IA-1.  |  Li, Q., et al. 1997. Biochem Biophys Res Commun. 236: 776-81. PMID: 9245732

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-INSM1 Antibody (A-8) has 126 citations in a variety of scientific publications.

Primary antibodies like Anti-INSM1 Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

INSM1 Antibody (A-8) Specifications

Alternate Product Name: INSM1

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 81-125

Epitope Target: INSM1

Epitope Species: human

Product Reactivity/Detection: INSM1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 126 citations in various scientific publications

Available Conjugates/Forms for INSM1 Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent