INSIG-1 Antibody (A-9): sc-390504

INSIG-1 Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects INSIG-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-INSIG-1 antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. INSIG-1 plays a crucial role in cholesterol homeostasis by functioning as a key regulator in the negative-feedback mechanism that inhibits cholesterol synthesis. INSIG-1 is primarily localized in the endoplasmic reticulum, where INSIG-1 interacts with the sterol-sensing domain of the SREBP cleavage-activating protein (SCAP). This interaction prevents SCAP export from the endoplasmic reticulum, thereby inhibiting the proteolytic processing of sterol regulatory element-binding proteins (SREBPs) in the Golgi apparatus. Oxysterols influence INSIG-1 regulation by suppressing gene expression, while agents like LY295427 can restore expression. INSIG-1′s importance in cholesterol metabolism highlights potential as a therapeutic target for conditions such as hypercholesterolemia, making INSIG-1 monoclonal antibody (A-9) a valuable tool for researchers studying lipid metabolism and related disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

INSIG-1 Antibody (A-9) References:

1. Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER.  |  Yang, T., et al. 2002. Cell. 110: 489-500. PMID: 12202038
2. Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins.  |  Yabe, D., et al. 2002. Proc Natl Acad Sci U S A. 99: 12753-8. PMID: 12242332
3. The hypocholesterolemic agent LY295427 up-regulates INSIG-1, identifying the INSIG-1 protein as a mediator of cholesterol homeostasis through SREBP.  |  Janowski, BA. 2002. Proc Natl Acad Sci U S A. 99: 12675-80. PMID: 12242342
4. Three mutations in sterol-sensing domain of SCAP block interaction with insig and render SREBP cleavage insensitive to sterols.  |  Yabe, D., et al. 2002. Proc Natl Acad Sci U S A. 99: 16672-7. PMID: 12482938
5. Hepatic insig-1 or -2 overexpression reduces lipogenesis in obese Zucker diabetic fatty rats and in fasted/refed normal rats.  |  Takaishi, K., et al. 2004. Proc Natl Acad Sci U S A. 101: 7106-11. PMID: 15096598
6. Juxtamembranous aspartic acid in Insig-1 and Insig-2 is required for cholesterol homeostasis.  |  Gong, Y., et al. 2006. Proc Natl Acad Sci U S A. 103: 6154-9. PMID: 16606821
7. The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.  |  Xu, D., et al. 2020. Nature. 580: 530-535. PMID: 32322062
8. Emerging role of Insig-1 in lipid metabolism and lipid disorders.  |  Ouyang, S., et al. 2020. Clin Chim Acta. 508: 206-212. PMID: 32461046
9. Hypomorphic ASGR1 modulates lipid homeostasis via INSIG1-mediated SREBP signaling suppression.  |  Xu, Y., et al. 2021. JCI Insight. 6: PMID: 34622799
10. Ubiquitination of NLRP3 by gp78/Insig-1 restrains NLRP3 inflammasome activation.  |  Xu, T., et al. 2022. Cell Death Differ. 29: 1582-1595. PMID: 35110683
11. A novel protein encoded by circINSIG1 reprograms cholesterol metabolism by promoting the ubiquitin-dependent degradation of INSIG1 in colorectal cancer.  |  Xiong, L., et al. 2023. Mol Cancer. 22: 72. PMID: 37087475
12. Cloning, human chromosomal assignment, and adipose and hepatic expression of the CL-6/INSIG1 gene.  |  Peng, Y., et al. 1997. Genomics. 43: 278-84. PMID: 9268630

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-INSIG-1 Antibody (A-9) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-INSIG-1 Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

INSIG-1 Antibody (A-9) Specifications

Alternate Product Name: INSIG1

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 31-100

Epitope Target: INSIG-1

Epitope Species: human

Product Reactivity/Detection: INSIG-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for INSIG-1 Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin