Date published: 2026-4-20

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IL-5 Antibody (B-2): sc-398334

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Datasheets
  • IL-5 Antibody (B-2) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 36-63 near the N-terminus of IL-5 of mouse origin
  • Anti-IL-5 Antibody (B-2) is recommended for detection of IL-5 of mouse and rat origin by WB, IP, IF and ELISA
  • Anti-IL-5 Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IL-5 Antibody (B-2) for WB applications. These reagents are now offered in bundles with IL-5 Antibody (B-2) (see ordering information below).
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IL-5 Antibody (B-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects IL-5 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IL-5 (B-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Interleukin-5, or IL-5, plays a crucial role in the immune system by promoting the growth and differentiation of B cells and eosinophils, which are essential for mounting an effective immune response, particularly in allergic reactions and asthma. IL-5 is primarily secreted by activated T cells and acts through the IL-5 receptor, which is composed of an alpha chain that binds IL-5 with low affinity and a beta chain that forms a high-affinity receptor complex. The beta chain is shared with the receptors for interleukin-3 and granulocyte-macrophage colony-stimulating factor, highlighting the interconnectedness of these signaling pathways in immune regulation. Understanding IL-5 and receptor function is vital for developing targeted therapies for allergic diseases and other conditions characterized by eosinophilia.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

IL-5 Antibody (B-2)

sc-398334
200 µg/ml
$322.00

IL-5 Antibody (B-2): m-IgG Fc BP-HRP Bundle

sc-530762
200 µg Ab; 10 µg BP
$361.00

IL-5 Antibody (B-2): m-IgGκ BP-HRP Bundle

sc-524297
200 µg Ab, 40 µg BP
$361.00

IL-5 Antibody (B-2): m-IgG2a BP-HRP Bundle

sc-547580
200 µg Ab; 10 µg BP
$361.00

IL-5 Antibody (B-2) AC

sc-398334 AC
500 µg/ml, 25% agarose
$424.00

IL-5 Antibody (B-2) HRP

sc-398334 HRP
200 µg/ml
$322.00

IL-5 Antibody (B-2) FITC

sc-398334 FITC
200 µg/ml
$336.00

IL-5 Antibody (B-2) PE

sc-398334 PE
200 µg/ml
$349.00

IL-5 Antibody (B-2) Alexa Fluor® 488

sc-398334 AF488
200 µg/ml
$364.00

IL-5 Antibody (B-2) Alexa Fluor® 546

sc-398334 AF546
200 µg/ml
$364.00

IL-5 Antibody (B-2) Alexa Fluor® 594

sc-398334 AF594
200 µg/ml
$364.00

IL-5 Antibody (B-2) Alexa Fluor® 647

sc-398334 AF647
200 µg/ml
$364.00

IL-5 Antibody (B-2) Alexa Fluor® 680

sc-398334 AF680
200 µg/ml
$364.00

IL-5 Antibody (B-2) Alexa Fluor® 790

sc-398334 AF790
200 µg/ml
$364.00

IL-5 (B-2) Neutralizing Peptide

sc-398334 P
100 µg/0.5 ml
$69.00

Is there a need of HRP linked secondary antibody to detect HRP tagged primary antibody of IL-5 or not. It can be detected using ECL or not?

Asked by: Ramiya
Thank you for your question. Direct HRP-conjugates such as sc-398334 HRP are designed to be used without a secondary antibody. Only one antibody incubation step is necessary. After this step your protein of interest can be detected using ECL as if you had used a secondary antibody.
Answered by: Tech Support Europe
Date published: 2019-07-24

What application is the blocking peptide sc-398334 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great Western blot data of mouse recombinant ILGreat Western blot data of mouse recombinant IL-5. -SCBT QC
Date published: 2013-03-28
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IL-5 Antibody (B-2) is rated 5.0 out of 5 by 1.
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