IL-3 Antibody (F-6): sc-398177

IL-3 Antibody (F-6) is a mouse monoclonal IgG2a antibody that detects interleukin-3 (IL-3) in mouse samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IL-3 is a pleiotropic cytokine primarily secreted by activated T lymphocytes, playing a crucial role in the proliferation and differentiation of hematopoietic cells. Anti-IL-3 antibody (F-6) supports research on pluripotent stem cells and differentiated progenitors while enhancing the functional activity of fully differentiated cells, making IL-3 vital for maintaining hematopoietic homeostasis. The IL-3 receptor, consisting of a ligand-specific α subunit and a common β subunit shared with IL-5 and GM-CSF receptors, is essential for mediating IL-3 biological effects. The carboxy terminus of the β subunit activates the MAP kinase signaling pathway, involved in various cellular processes. IL-3 receptor stimulation leads to tyrosine phosphorylation of JAK2, a member of the JAK/Tyk 2 family, subsequently activating Stat5a and Stat5b, which translocate to the nucleus to regulate gene expression. IL-3 monoclonal antibody (F-6) serves as an invaluable tool for researchers studying hematopoiesis and signaling pathways involved in immune responses.

The preferred IL-3 antibody is IL-3 Antibody (G-1): sc-28342.