Anti-IL-3 Antibody (G-1) is a mouse monoclonal IgG1 (kappa light chain) IL-3 antibody provided at 200 µg/ml
raised against amino acids 20-152 of IL-3 of human origin
IL-3 Antibody (G-1) is recommended for detection of IL-3 of human origin by WB, IP, IF and ELISA
Anti-IL-3 Antibody (G-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IL-3 (G-1): sc-28342.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
IL-3 Antibody (G-1) is a high quality monoclonal IL-3 antibody (also designated IL-3 antibody) suitable for the detection of the IL-3 protein of human origin. IL-3 Antibody (G-1) is available as both the non-conjugated anti-IL-3 antibody form, as well as multiple conjugated forms of anti-IL-3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL-3 has also been shown to protect mast cells from undergoing apoptosis. IL-3 exerts its biological effects through a receptor which consists of a ligand-specific α subunit and a signal transducing β subunit common to the IL-3/IL-5/GM-CSF receptors. The carboxy terminus of the β subunit has been shown to be necessary for activation of the MAP kinase signaling pathway. Although the IL-3 receptor has no intrinsic kinase activity, stimulation with IL-3 leads to tyrosine phosphorylation of the JAK/Tyk 2 family member, JAK2, which in turn activates and causes nuclear translocation of Stat5a and Stat5b.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
For Western Blot, is it recommended to use denatured or non-denatured conditions with IL-3 (G-1): sc-28342 monoclonal antibody?
Asked by: cjMara
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Rated 5 out of
it works very wellI use Western blotting detection lymphocytes, 1:1000 diluted, it works very well.
Date published: 2017-05-22
Rated 5 out of
incredible IL-3 Antibody (G-1)This is incredible IL-3 Antibody (G-1).Used the HRP form in WB and worked beautifully.Strong, clear, specific band in WB in bovine cells.I recommend you buy this antibody.
Date published: 2016-12-21
Rated 5 out of
Produced positive Western Blot data of humanProduced positive Western Blot data of human recombinant IL-3. -SCBT QC
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn