Date published: 2025-9-5

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IL-10 抗体 (E-10): sc-8438

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说明书
  • IL-10 抗体 E-10 是小鼠单克隆 IgG2b κ,IL-10抗体, 在71篇文献中引用,规格为200 µg/ml
  • 针对氨基酸19-178的抗原,代表成熟IL-10,来源于human
  • IL-10 抗体 (E-10) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 IL-10
  • 抗IL-10抗体(E-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IL-10 (E-10): sc-8438免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRPm-IgGκ BP-HRP 是 IL-10 抗体 (E-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与IL-10 抗体 (E-10) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

IL-10抗体(E-10)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过WB、IP、IF、IHCP和ELISA检测人类IL-10蛋白。IL-10抗体(E-10)有非结合型和多种结合型,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合物。白细胞介素-10(IL-10)是一种主要由TH2克隆分泌的178个氨基酸的蛋白质,在免疫调节中起着至关重要的作用。它的主要功能之一是抑制TH1细胞产生促炎细胞因子,这对于维持免疫平衡和防止过度炎症反应至关重要,过度炎症反应会导致组织损伤。此外,IL-10通过下调CD25(IL-2受体)来抑制巨噬细胞和B淋巴细胞的活化,从而调节免疫反应。IL-10在免疫调节中的重要性在各种病理条件下得到了强调,包括HIV-1感染,其中IL-10与疾病进展有关。人类和鼠类IL-10的序列高度相似,氨基酸水平上相似度为81%,两种形式在溶液中都以非共价键合的同二聚体形式存在,通过IL-10受体发挥生物学效应,IL-10受体是一种糖蛋白,可通过脂多糖刺激诱导巨噬细胞和成纤维细胞产生。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IL-10 抗体 (E-10) 参考文献:

  1. 乳糜泻患者的人类肠道字母 IEL 克隆显示 IL-10 合成减少,IL-2 生成增强。  |  Kolkowski, EC., et al. 2006. Cell Immunol. 244: 1-9. PMID: 17368439
  2. IL-10转导的骨髓间充质干细胞可减轻实验性异体干细胞移植后急性移植物抗宿主疾病的严重程度。  |  Min, CK., et al. 2007. Bone Marrow Transplant. 39: 637-45. PMID: 17369865
  3. 免疫细胞对IL-10产生的调节。  |  Saraiva, M. and O'Garra, A. 2010. Nat Rev Immunol. 10: 170-81. PMID: 20154735
  4. IL-10家族细胞因子在炎症和疾病中的调节和功能。  |  Ouyang, W., et al. 2011. Annu Rev Immunol. 29: 71-109. PMID: 21166540
  5. IL-10抑制鞘脂代谢以限制炎症。  |  York, AG., et al. 2024. Nature. 627: 628-635. PMID: 38383790
  6. IL-10 可增强 T 细胞 IL-2 受体α链的表达。  |  Cohen, SB., et al. 1994. Immunology. 83: 329-32. PMID: 7835955
  7. 脂多糖依赖性诱导小鼠成纤维细胞表达 IL-10 受体。  |  Weber-Nordt, RM., et al. 1994. J Immunol. 153: 3734-44. PMID: 7930590
  8. 大鼠细胞因子合成抑制因子(IL-10)cDNA的分子克隆及在脾脏和巨噬细胞中的表达。  |  Feng, L., et al. 1993. Biochem Biophys Res Commun. 192: 452-8. PMID: 8484757
  9. IL-10 与 TNF-α 协同激活潜伏和急性感染的单核细胞/巨噬细胞系细胞中的 HIV-1。  |  Finnegan, A., et al. 1996. J Immunol. 156: 841-51. PMID: 8543841
  10. HIV-1从生产性感染的成熟朗格汉斯细胞向初级CD4+T淋巴细胞传播,导致T细胞反应改变,IFN-gamma和IL-10的产生增强。  |  Ludewig, B., et al. 1996. Virology. 215: 51-60. PMID: 8553586
  11. 巨噬细胞细胞表面的 IL-10 可调节巨噬细胞的杀菌活性。  |  Fleming, SD. and Campbell, PA. 1996. J Immunol. 156: 1143-50. PMID: 8557991
  12. 移植中的 IL-10 免疫抑制。  |  Bromberg, JS. 1995. Curr Opin Immunol. 7: 639-43. PMID: 8573306

订购信息

产品名称产品编号规格价格数量收藏夹

IL-10 抗体 (E-10)

sc-8438
200 µg/ml
$316.00

IL-10 (E-10): m-IgG Fc BP-HRP 套装

sc-528235
200 µg Ab; 10 µg BP
$354.00

IL-10 (E-10): m-IgGκ BP-HRP 套装

sc-520561
200 µg Ab, 40 µg BP
$354.00

IL-10 (E-10): m-IgG2b BP-HRP 套装

sc-550011
200 µg Ab; 10 µg BP
$354.00

IL-10 抗体 (E-10) AC

sc-8438 AC
500 µg/ml, 25% agarose
$416.00

IL-10 抗体 (E-10) HRP

sc-8438 HRP
200 µg/ml
$316.00

IL-10 抗体 (E-10) FITC

sc-8438 FITC
200 µg/ml
$330.00

IL-10 抗体 (E-10) PE

sc-8438 PE
200 µg/ml
$343.00

IL-10 抗体 (E-10) Alexa Fluor® 488

sc-8438 AF488
200 µg/ml
$357.00

IL-10 抗体 (E-10) Alexa Fluor® 546

sc-8438 AF546
200 µg/ml
$357.00

IL-10 抗体 (E-10) Alexa Fluor® 594

sc-8438 AF594
200 µg/ml
$357.00

IL-10 抗体 (E-10) Alexa Fluor® 647

sc-8438 AF647
200 µg/ml
$357.00

IL-10 抗体 (E-10) Alexa Fluor® 680

sc-8438 AF680
200 µg/ml
$357.00

IL-10 抗体 (E-10) Alexa Fluor® 790

sc-8438 AF790
200 µg/ml
$357.00

Este anticuerpo funciona para determinar IL-10 en conejos? Does this antibody work to determine IL-10 in rabbits?

Asked by: Juan Pablo Rodríguez
Muchas gracias por su pregunta. No recomendamos utilizar este anticuerpo en conejo. Si necesita cualquier información adicional, por favor contacte con nuestro servivio Técnico: scbt@scbt.com, (800) 457-3801
Answered by: Technical Support Europe
Date published: 2017-09-21

What is the recommended secondary to use with this primary?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 3 out of 5 by from Works well for WBLoaded 30ug of mouse neonatal lung lysates and used the antibody at a dilution of 1:500
Date published: 2019-10-22
Rated 5 out of 5 by from Effect is Very GoodKnowledge of the sensitivity and specificity of IL-10 Antibody leads to their more rational selection.It with a precise position effect and very powerful signal.
Date published: 2017-10-16
Rated 5 out of 5 by from availableI use the antibody in tubular epithelial cells.Its result is good for me .I will continue to use it for my experiment.
Date published: 2017-10-01
Rated 3 out of 5 by from Good antibodyWorked okay with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 1 out of 5 by from IHC Assay Review for IL-10 mABApplication: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: No staining observed.
Date published: 2017-08-31
Rated 5 out of 5 by from 染色好IL10抗体效果好,免疫组化染色均匀,阳性着色明显。推荐大家购买,物美价廉,用的1:50用的非常不错。
Date published: 2017-07-21
Rated 2 out of 5 by from Incorrect amount sentHi I ordered this MAb as a free sample and the vial said 10 ug in 50 ul. I used it once for a western blot (15ul) that ended up not working (probably for my own reasons though). Then when I went to try again, there was none left. Not sure if the full 50 ul was supplied, but it was extremely disappointing to have done all the work of making a second gel and running it only to find that I didn't have enough antibody.
Date published: 2017-06-29
Rated 5 out of 5 by from clean bandThe antibody used for detect IL-10 by WB,we can easily got the band at 56kda,very clean ,no background.
Date published: 2017-06-24
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IL-10 抗体 (E-10) is rated 4.3 out of 5 by 18.
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