IKK gamma Antibody (1.T.26) recommended for detection of IKKγ of human origin by WB and IP

IKKγ Antibody (1.T.26): sc-71331

IKKγ Antibody (1.T.26) is rated 5.0 out of 5 by 3.
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Datasheets

    • IKKγ Antibody (1.T.26) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against full length His-tagged IKKγ of human origin
    • recommended for detection of IKKγ of human origin by WB and IP
    • See IKKγ (F-10): sc-166398 for IKKγ antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using IKKγ (1.T.26): sc-71331 antibody for immunoprecipitation, how much antibody should I use?

Asked by: jenni
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good data for WB in non Good data for WB in non-transfected 293(A), human IKK transfected 293(B) and Jurkat (C) whole cell lysates. -SCBT QC
Date published: 2015-05-08
Rated 5 out of 5 by from Worked well with non Worked well with non-transfected 293T (A), human IKK transfected 293T(B) and K-562 (C) whole cell lysates. -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from Single strong band for WB in BJAB (A) Single strong band for WB in BJAB (A), Jurkat (B), K-562 (C), U-937 (D), SW-13 (E) and HeLa (F) whole cell lysates. -SCBT QC
Date published: 2013-09-01
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