IKK alpha Antibody (C-6) recommended for detection of IKKα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

IKKα Antibody (C-6): sc-166231

IKKα Antibody (C-6) is rated 5.0 out of 5 by 3.
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Datasheets

    • IKKα Antibody (C-6) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 248-452 mapping at the C-terminus of IKKα of human origin
    • recommended for detection of IKKα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See IKKα (B-8): sc-7606 for IKKα antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using Ikka (C-6): sc-166231 to stain fixed frozen sections. Do you recommend antigen retrieval?

Asked by: signalingJoan
Thank you for your question. Yes, we recommend antigen retrieval for this antibody. You can find the recommended protocol, including antigen retrieval, here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-03-26

I am using IKKα (C-6): sc-166231 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmic Produced excellent immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Publication in WB with mouse renal tissue Publication in WB with mouse renal tissue. -SCBT QC
Date published: 2015-04-11
Rated 5 out of 5 by from Works great for Western in Jurkat whole cell Works great for Western in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-03-03
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