IKK-ε Antibody (A-11): sc-376114

IKK-ε Antibody (A-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects IKK-ε protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). IKK-ε (A-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IKK-ε protein, also known as IKBKE or IKK-i, is a serine/threonine kinase that plays a crucial role in immune response by regulating the activation of transcription factor NFκB. IKK-ε protein is primarily expressed in immune cells and is induced by stimuli such as lipopolysaccharide and proinflammatory cytokines like TNFα, IL-1, and IL-6. IKK-ε protein is essential for phosphorylation of IκBα at specific serine residues, which leads to degradation of IκB and subsequent release and activation of NFκB. IKK-ε protein′s ability to modulate NFκB signaling is significant, as NFκB is involved in various cellular processes, including inflammation, immune response, and cell survival. Overexpression of IKK-ε protein has been linked to enhanced phosphorylation of IκBα and increased NFκB activity, indicating a potential role as an IκB kinase in immune regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

IKK-ε Antibody (A-11) References:

1. IKKε aggravates inflammatory response via activation of NF-κB in rheumatoid arthritis.  |  Zhou, LF., et al. 2018. Eur Rev Med Pharmacol Sci. 22: 2126-2133. PMID: 29687872
2. Inhibition of TBK1/IKKε Promotes Regeneration of Pancreatic β-cells.  |  Xu, J., et al. 2018. Sci Rep. 8: 15587. PMID: 30349097
3. IKKε isoform switching governs the immune response against EV71 infection.  |  Chang, YL., et al. 2021. Commun Biol. 4: 663. PMID: 34079066
4. Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation.  |  Verma, IM., et al. 1995. Genes Dev. 9: 2723-35. PMID: 7590248
5. NF-kappa B: a lesson in family values.  |  Thanos, D. and Maniatis, T. 1995. Cell. 80: 529-32. PMID: 7867060
6. CHUK, a new member of the helix-loop-helix and leucine zipper families of interacting proteins, contains a serine-threonine kinase catalytic domain.  |  Connelly, MA. and Marcu, KB. 1995. Cell Mol Biol Res. 41: 537-49. PMID: 8777433
7. MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1.  |  Malinin, NL., et al. 1997. Nature. 385: 540-4. PMID: 9020361
8. Identification and characterization of an IkappaB kinase.  |  Régnier, CH., et al. 1997. Cell. 90: 373-83. PMID: 9244310
9. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB.  |  DiDonato, JA., et al. 1997. Nature. 388: 548-54. PMID: 9252186
10. Tumor necrosis factor (TNF)-mediated kinase cascades: bifurcation of nuclear factor-kappaB and c-jun N-terminal kinase (JNK/SAPK) pathways at TNF receptor-associated factor 2.  |  Song, HY., et al. 1997. Proc Natl Acad Sci U S A. 94: 9792-6. PMID: 9275204
11. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation.  |  Zandi, E., et al. 1997. Cell. 91: 243-52. PMID: 9346241
12. Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation.  |  Yamaoka, S., et al. 1998. Cell. 93: 1231-40. PMID: 9657155

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IKK-ε Antibody (A-11) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-IKK-ε Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IKK-ε Antibody (A-11) Specifications

Alternate Product Name: IKK-ε

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 2-24

Epitope Target: IKK-ε

Epitope Species: human

Product Reactivity/Detection: IKK-ε

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for IKK-ε Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin