NFKBIA/IkB alpha Antibody (H-4): sc-1643

IκB-α Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects IκB-α in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). IκB-α plays a crucial role in the regulation of the NF-κB signaling pathway, which is essential for controlling immune responses, inflammation, and cell survival. By sequestering NF-κB dimers in the cytoplasm, IκB-α prevents their translocation to the nucleus, thereby inhibiting the expression of target genes involved in these processes. IκB-α (H-4) antibody maintains cellular homeostasis and prevents excessive inflammatory responses, which can lead to various diseases, including cancer and autoimmune disorders. IκB-α (H-4) monoclonal antibody is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates, making IκB-α (H-4) antibody a versatile tool for researchers studying the NF-κB pathway and its implications in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NFKBIA/IkB alpha Antibody (H-4) References:

1. I kappa B gamma, a 70 kd protein identical to the C-terminal half of p110 NF-kappa B: a new member of the I kappa B family.  |  Inoue, J., et al. 1992. Cell. 68: 1109-20. PMID: 1339305
2. Exposure to hydrogen peroxide diminishes NF-kappaB activation, IkappaB-alpha degradation, and proteasome activity in neutrophils.  |  Zmijewski, JW., et al. 2007. Am J Physiol Cell Physiol. 293: C255-66. PMID: 17392377
3. Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity.  |  Haskill, S., et al. 1991. Cell. 65: 1281-9. PMID: 1829648
4. Rel-associated pp40: an inhibitor of the rel family of transcription factors.  |  Davis, N., et al. 1991. Science. 253: 1268-71. PMID: 1891714
5. The rel-associated pp40 protein prevents DNA binding of Rel and NF-kappa B: relationship with I kappa B beta and regulation by phosphorylation.  |  Kerr, LD., et al. 1991. Genes Dev. 5: 1464-76. PMID: 1907941
6. Activation in vitro of NF-kappa B by phosphorylation of its inhibitor I kappa B.  |  Ghosh, S. and Baltimore, D. 1990. Nature. 344: 678-82. PMID: 2157987
7. I kappa B-beta regulates the persistent response in a biphasic activation of NF-kappa B.  |  Thompson, JE., et al. 1995. Cell. 80: 573-82. PMID: 7867065
8. I kappa B epsilon, a novel member of the I kappa B family, controls RelA and cRel NF-kappa B activity.  |  Whiteside, ST., et al. 1997. EMBO J. 16: 1413-26. PMID: 9135156
9. Cloning and functional characterization of mouse IkappaBepsilon.  |  Simeonidis, S., et al. 1997. Proc Natl Acad Sci U S A. 94: 14372-7. PMID: 9405619

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NFKBIA/IkB alpha Antibody (H-4) has 558 citations in a variety of scientific publications.

Primary antibodies like Anti-NFKBIA/IkB alpha Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NFKBIA/IkB alpha Antibody (H-4) Specifications

Alternate Product Name: IκB-α

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-317

Epitope Target: IκB-α

Epitope Species: human

Product Reactivity/Detection: IκB-α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 558 citations in various scientific publications

Available Conjugates/Forms for NFKBIA/IkB alpha Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine

The preferred p-NFKBIA/IkB alpha antibody is p-NFKBIA/IkB alpha Antibody (B-9): sc-8404.