IgA Antibody (A-9): sc-373823

IgA Antibody (A-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects IgA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IgA (A-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Immunoglobulin A (IgA) plays a crucial role in the mucosal immune system, primarily functioning to protect mucosal surfaces from pathogens. IgA is predominantly produced by B cells located in gut-associated lymphoid tissues, with daily production surpassing that of other immunoglobulins. The structure of IgA typically exists as dimers or polymers, formed by the association of two monomeric units linked by a joining (J) chain. This dimeric or polymeric form is essential for function, allowing IgA to effectively bind to the polymeric immunoglobulin receptor (pIgR), facilitating transport across epithelial cells to mucosal surfaces where IgA can neutralize pathogens and prevent their entry into the body. The heavy chain of IgA is an α-chain, while the light chains can be either κ- or λ- chains, contributing to the diversity and specificity of the immune response.

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IgA Antibody (A-9) References:

1. Differentiated B lymphocytes. Potential to express particular antibody variable and constant regions depends on site of lymphoid tissue and antigen load.  |  Gearhart, PJ. and Cebra, JJ. 1979. J Exp Med. 149: 216-27. PMID: 105075
2. The J chain is essential for polymeric Ig receptor-mediated epithelial transport of IgA.  |  Johansen, FE., et al. 2001. J Immunol. 167: 5185-92. PMID: 11673531
3. The carboxyl-terminal domains of IgA and IgM direct isotype-specific polymerization and interaction with the polymeric immunoglobulin receptor.  |  Braathen, R., et al. 2002. J Biol Chem. 277: 42755-62. PMID: 12213814
4. Structural requirements for the interaction of human IgA with the human polymeric Ig receptor.  |  Lewis, MJ., et al. 2005. J Immunol. 175: 6694-701. PMID: 16272325
5. Generation of gut-homing IgA-secreting B cells by intestinal dendritic cells.  |  Mora, JR., et al. 2006. Science. 314: 1157-60. PMID: 17110582
6. [IgA, an essential part of the immune system: selected issues].  |  Czyzewska-Buczyńska, A., et al. 2006. Postepy Hig Med Dosw (Online). 61: 38-47. PMID: 17369772
7. Secretory immunoglobulin A (IgA) responses in IgA nephropathy patients after mucosal immunization, as part of a polymeric IgA response.  |  Eijgenraam, JW., et al. 2008. Clin Exp Immunol. 152: 227-32. PMID: 18336594
8. The molecular organization of the protein HC-IgA complex (HC-IgA).  |  Grubb, A., et al. 1986. J Biol Chem. 261: 14313-20. PMID: 2429955
9. IgA tracheobronchial deposits underlie respiratory compromise in neonatal linear IgA bullous dermatosis.  |  Diociaiuti, A., et al. 2017. J Eur Acad Dermatol Venereol. 31: e333-e335. PMID: 28079922
10. Specificity of immunoglobulin heavy chain switch correlates with activity of germline heavy chain genes prior to switching.  |  Stavnezer-Nordgren, J. and Sirlin, S. 1986. EMBO J. 5: 95-102. PMID: 3007121
11. Characteristics of IgA and macromolecular IgA in sera from IgA nephropathy transplanted patients with and without IgAN recurrence.  |  Coppo, R., et al. 1995. Contrib Nephrol. 111: 85-92. PMID: 7758351
12. Human triclonal anti-IgG gammopathy. II. Determination of the antigenic specificity patterns of the IgG, IgA and IgM autoantibodies for the subclasses of IgG.  |  Abraham, GN., et al. 1978. Immunology. 35: 437-45. PMID: 81177

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IgA Antibody (A-9) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-IgA Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IgA Antibody (A-9) Specifications

Alternate Product Name: IgA

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 265-301

Epitope Target: IgA

Epitope Species: human

Product Reactivity/Detection: IgA₁ and IgA₂

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for IgA Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin