Ig kappa chain Antibody (OX12) recommended for detection of a determinant on rat κ chains of mouse and rat origin by IF and FCM

Ig κ chain Antibody (OX12): sc-53079

Ig κ chain Antibody (OX12) is rated 3.0 out of 5 by 1.
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Datasheets

    • Ig κ chain Antibody (OX12) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against F(ab')2 fragment of IgG of rat origin
    • recommended for detection of a determinant on rat κ chains of mouse and rat origin by IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How can Ig κ chain (OX12): sc-53079 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Professor Griffin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-53079 PE) or fluoroscein (sc-53079 FITC).
Answered by: Technical Support
Date published: 2017-03-01
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Rated 3 out of 5 by from Produced decent indirect FCM data for rat Produced decent indirect FCM data for rat peripheral blood leukocytes stained Ig k chain . -SCBT QC
Date published: 2013-03-01
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