Ig κ chain Antibody (L1C1): sc-59265
- Ig κ chain Antibody (L1C1) is a mouse monoclonal IgG1 κ Ig κ chain antibody provided at 200 µg/ml
- raised against B lymphoma cells of human origin
- Ig kappa chain Antibody (L1C1) is recommended for detection of Ig κ chain of human origin by WB, IP and FCM
- Anti-Ig kappa chain Antibody (L1C1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Ig κ chain Antibody (L1C1) for WB applications. These reagents are now offered in bundles with Ig κ chain Antibody (L1C1) (see ordering information below).
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Ig κ chain Antibody (L1C1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ig κ chain protein of human origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). Ig κ chain (L1C1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ig κ chain plays a crucial role in immune response, as one of two types of light chains forming part of immunoglobulin structure, essential for specificity and functionality of antibodies. Immunoglobulins, composed of two identical heavy chains and two identical light chains, are vital for recognizing and binding to antigens, facilitating their removal from the body. Kappa light chain presence influences overall stability and affinity of antibodies for target antigens. In humans, kappa light chain genes are located on chromosome 2, and their rearrangement during B cell development creates diverse antibody repertoires capable of responding to various pathogens. Unique genetic combinations of heavy and light chains enable production of antibodies with specific binding sites, fundamental for effective immune responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ig κ chain Antibody (L1C1) References:
- Nucleotide sequence analysis of a transforming gene isolated from nasopharyngeal carcinoma cell line CNE2: an aberrant human immunoglobulin kappa light chain which lacks variable region. | Li, M., et al. 2001. DNA Seq. 12: 331-5. PMID: 11913778
- Epstein-Barr Virus Encoded Latent Membrane Protein 1 Increases Expression of Immunoglobulin kappa Light Chain through NFkappaB in a Nasopharyngeal Carcinoma Cell Line. | Liao, W., et al. 1999. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 31: 659-663. PMID: 12110931
- Immunoglobulin kappa light chain germ-line transcripts in human precursor B lymphocytes. | Thompson, A., et al. 1992. Eur J Immunol. 22: 3167-71. PMID: 1446707
- The immunoglobulin kappa light chain repertoire expressed in the synovium of a patient with rheumatoid arthritis. | Lee, SK., et al. 1992. Arthritis Rheum. 35: 905-13. PMID: 1642656
- Diagnostic value of immunoglobulin κ light chain gene rearrangement analysis in B-cell lymphomas. | Kokovic, I., et al. 2015. Int J Oncol. 46: 953-62. PMID: 25501347
- Dynamic Control of Long-Range Genomic Interactions at the Immunoglobulin κ Light-Chain Locus. | Ribeiro de Almeida, C., et al. 2015. Adv Immunol. 128: 183-271. PMID: 26477368
- A labile inhibitor blocks immunoglobulin kappa-light-chain-gene transcription in a pre-B leukemic cell line. | Wall, R., et al. 1986. Proc Natl Acad Sci U S A. 83: 295-8. PMID: 3079910
- Dispersed human immunoglobulin kappa light-chain genes. | Lötscher, E., et al. Nature. 320: 456-8. PMID: 3083265
- Accumulation of wild-type p53 protein upon gamma-irradiation induces a G2 arrest-dependent immunoglobulin kappa light chain gene expression. | Aloni-Grinstein, R., et al. 1995. EMBO J. 14: 1392-401. PMID: 7729417
- Detection of immunoglobulin kappa light-chain gene rearrangement patterns by Southern blot analysis. | Beishuizen, A., et al. 1994. Leukemia. 8: 2228-36; discussion 2237-9. PMID: 7808012
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ig κ chain Antibody (L1C1) | sc-59265 | 200 µg/ml | $316.00 | |||
Ig κ chain Antibody (L1C1): m-IgG Fc BP-HRP Bundle | sc-526925 | 200 µg Ab; 10 µg BP | $354.00 | |||
Ig κ chain Antibody (L1C1): m-IgG1 BP-HRP Bundle | sc-532298 | 200 µg Ab; 20 µg BP | $354.00 | |||
Ig κ chain Antibody (L1C1) AC | sc-59265 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Ig κ chain Antibody (L1C1) HRP | sc-59265 HRP | 200 µg/ml | $316.00 | |||
Ig κ chain Antibody (L1C1) FITC | sc-59265 FITC | 200 µg/ml | $330.00 | |||
Ig κ chain Antibody (L1C1) PE | sc-59265 PE | 200 µg/ml | $343.00 | |||
Ig κ chain Antibody (L1C1) Alexa Fluor® 488 | sc-59265 AF488 | 200 µg/ml | $357.00 | |||
Ig κ chain Antibody (L1C1) Alexa Fluor® 546 | sc-59265 AF546 | 200 µg/ml | $357.00 | |||
Ig κ chain Antibody (L1C1) Alexa Fluor® 594 | sc-59265 AF594 | 200 µg/ml | $357.00 | |||
Ig κ chain Antibody (L1C1) Alexa Fluor® 647 | sc-59265 AF647 | 200 µg/ml | $357.00 | |||
Ig κ chain Antibody (L1C1) Alexa Fluor® 680 | sc-59265 AF680 | 200 µg/ml | $357.00 | |||
Ig κ chain Antibody (L1C1) Alexa Fluor® 790 | sc-59265 AF790 | 200 µg/ml | $357.00 |