IFN-alpha Antibody (F-7) recommended for detection of IFN-α1 and IFN-α13 of human origin by WB, IP, IF, IHC(P) and ELISA

IFN-α Antibody (F-7): sc-373757

IFN-α Antibody (F-7) is rated 3.8 out of 5 by 4.
  • y_2020, m_8, d_9, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.10
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_373757, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 137ms
  • REVIEWS, PRODUCT
5
2
4
1
3
0
2
0
1
1

Datasheets

    • Anti-IFN-α Antibody (F-7) is a mouse monoclonal IgG2b (kappa light chain) IFN-α antibody provided at 200 µg/ml
    • raised against full length recombinant IFN-α1/13 of human origin
    • IFN-alpha Antibody (F-7) is recommended for detection of IFN-α1 and IFN-α13 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-IFN-alpha Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IFN-α (F-7): sc-373757.

I read the first review that the researcher use this product for fixed frozen mouse tissues for IF; thus, is it possible use this antibody for western-blot of mouse IFN alpha?

Asked by: jessie011
Thank you for your question. We have not yet tested this antibody in mouse samples, and so it is not recommended for detection in mouse at this time. The exact epitope recognized by this monoclonal antibody has not yet been determined. The full-length human antigen sequence appears to share approximately 65% sequence identity with some of IFN alpha proteins in mouse. It may cross-react, or it may not, but this would have to be determined experimentally.
Answered by: Technical Support
Date published: 2020-05-14

I am using IFN-α (F-7): sc-373757 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenni
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2019-03-01
  • y_2020, m_8, d_9, h_16CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.10
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_373757, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 127ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Good antibody This antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 1 out of 5 by from No endogenous IFNalpha in WB detectable There's no signal of endogenous IFN alpha in stimulated human fibroblasts detectable, even with a chemiluminescent femto kit
Date published: 2017-08-01
Rated 5 out of 5 by from very good I have just use this antibody in IFN-α for human hippocampal neurons,use PFA fixed. It works very nice. Beautiful surface staining of neurites.IF dilution 1:200 dilution, The result is very good.
Date published: 2017-07-29
Rated 5 out of 5 by from Great Western blot data of human recombinant IFN Great Western blot data of human recombinant IFN- . -SCBT QC
Date published: 2014-06-27
  • y_2020, m_8, d_9, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.10
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_373757, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 23ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.