IFIT3 Antibody (B-7) recommended for detection of IFIT3 of human origin by WB, IP, IF, IHC(P) and ELISA

IFIT3 Antibody (B-7): sc-393512

IFIT3 Antibody (B-7) is rated 5.0 out of 5 by 7.
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Datasheets

    • Anti-IFIT3 Antibody (B-7) is a mouse monoclonal IgG1 (kappa light chain) IFIT3 antibody provided at 200 µg/ml
    • raised against amino acids 286-490 mapping at the C-terminus of IFIT3 of human origin
    • IFIT3 Antibody (B-7) is recommended for detection of IFIT3 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-IFIT3 Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IFIT3 (B-7): sc-393512.

What is the recommended starting dilution for Western blot for sc-393512: IFIT3 (B-7) monoclonal antibody?

Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22

Hello, I was wondering if this product detects the 2 protein isoforms (a and b)?

Asked by: nmn55
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Rated 5 out of 5 by from Recognizes IFIT3 nicely in WB. Quality depends on applied blocking reagent. Good signals in whole cell lysates of HeLa cells using 1:1000 dilution and blocking in Rotiblock.
Date published: 2018-04-09
Rated 5 out of 5 by from Good antibody It detected endogenous IFIT3 in immortalized HFFs (1:500 dilution in 5% milk in TBS-0.1% Tween). Recommended!
Date published: 2017-10-28
Rated 5 out of 5 by from Works well in Western Blot Used at 1:1000 dilution overnight with samples from cultured cells and human tissues using 5-15 ug protein per lane. Very clean bands in both cultured cells and human tissue.
Date published: 2017-09-28
Rated 5 out of 5 by from IFIT3 (B-7) Excelent antibody for western blot. Detected IFIT3 at expected size in 15 ug total proteine lysate, wet transfer one hour at 450 mAmps on PVDF membrane, overnight blocking with 5% nonfat dry milk in PBS, one hour incubation at RT with 1:500 anti IFIT3 in 5% PBS with 0,2%Tween followed by one hour incubation with 1:10.000 anti mouse (sc-358914) HRP conjugated antibody and detection with ECL prime substrate followed by short film exposure (under 1 minute) and automatic processing.
Date published: 2017-09-25
Rated 5 out of 5 by from strong band Precise band acquired in WB analysis,recommend use it for 1:1000 dilution.
Date published: 2017-02-03
Rated 5 out of 5 by from Good for WB This antibody shows good band strength and detection of IFIT3 in multiple cell lines in Western Blot
Date published: 2017-01-31
Rated 5 out of 5 by from Very good Western blot data of IFIT3 expression Very good Western blot data of IFIT3 expression in Caki-1, AML-193, HeLa and Hep G2 whole cell lysates. -SCBT QC
Date published: 2014-02-02
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