IDH1/2 Antibody (G-11): sc-373816
- IDH1/2 Antibody (G-11) is a mouse monoclonal IgG1 κ IDH1/2 antibody, cited in 4 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 123-154 within an internal region of IDH2 of human origin
- IDH1/2 Antibody (G-11) is recommended for detection of IDH1 and IDH2 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-IDH1/2 Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IDH1/2 Antibody (G-11) for WB and IHC(P) applications. These reagents are now offered in bundles with IDH1/2 Antibody (G-11) (see ordering information below).
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IDH1/2 Antibody (G-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects IDH1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). IDH1/2 (G-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IDH2 protein, also known as isocitrate dehydrogenase 2 (NADP+, mitochondrial), is a crucial enzyme composed of 452 amino acids, encoded by the human gene IDH2. IDH2 is integral to cellular metabolism, catalyzing the conversion of isocitrate to alpha-ketoglutarate while reducing NADP+ to NADPH, which maintains cellular redox state and supports glutathione synthesis, a key antioxidant. IDH2 functions are essential for energy production and intermediary metabolism, with dysregulation implicated in various metabolic disorders and cancers. IDH2 localizes in the mitochondrion, forming a homodimer and binding to either magnesium or manganese ions, which are critical for enzymatic activity. IDH2′s close association with the pyruvate dehydrogenase complex links carbohydrate metabolism to the tricarboxylic acid cycle, highlighting IDH2′s importance in cellular energy homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IDH1/2 Antibody (G-11) References:
- Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation. | Figueroa, ME., et al. 2010. Cancer Cell. 18: 553-67. PMID: 21130701
- Isocitrate dehydrogenase 2 inhibits gastric cancer cell invasion via matrix metalloproteinase 7. | Wu, D. 2016. Tumour Biol. 37: 5225-30. PMID: 26553362
- Enasidenib in mutant IDH2 relapsed or refractory acute myeloid leukemia. | Stein, EM., et al. 2017. Blood. 130: 722-731. PMID: 28588020
- Isocitrate dehydrogenase type 2 (IDH2) is part of a multiprotein complex for placental steroidogenesis. | Urban-Sosa, VA., et al. 2019. Placenta. 87: 30-37. PMID: 31542634
- The prognostic significance of wild-type isocitrate dehydrogenase 2 (IDH2) in breast cancer. | Aljohani, AI., et al. 2020. Breast Cancer Res Treat. 179: 79-90. PMID: 31599393
- Isocitrate dehydrogenase 2 deficiency aggravates prolonged high-fat diet intake-induced hypertension. | Noh, MR., et al. 2020. Redox Biol. 34: 101548. PMID: 32388270
- Therapy for isocitrate dehydrogenase 2 (IDH2)R172 -mutant acute myeloid leukaemia. | Linch, DC., et al. 2022. Br J Haematol. 196: 1348-1352. PMID: 34870324
- IDH2 stabilizes HIF-1α-induced metabolic reprogramming and promotes chemoresistance in urothelial cancer. | Shigeta, K., et al. 2023. EMBO J. 42: e110620. PMID: 36637036
- Wild-type IDH2 is a therapeutic target for triple-negative breast cancer. | Li, JJ., et al. 2024. Nat Commun. 15: 3445. PMID: 38658533
- Epigenetic dysregulation in cancers by isocitrate dehydrogenase 2 (IDH2). | Nayarisseri, A., et al. 2024. Adv Protein Chem Struct Biol. 141: 223-253. PMID: 38960475
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IDH1/2 Antibody (G-11) | sc-373816 | 200 µg/ml | $322.00 | |||
IDH1/2 Antibody (G-11): m-IgG Fc BP-HRP Bundle | sc-529576 | 200 µg Ab; 10 µg BP | $361.00 | |||
IDH1/2 Antibody (G-11): m-IgGκ BP-HRP Bundle | sc-522514 | 200 µg Ab, 40 µg BP | $361.00 | |||
IDH1/2 Antibody (G-11): m-IgG1 BP-HRP Bundle | sc-543591 | 200 µg Ab; 20 µg BP | $361.00 | |||
IDH1/2 Antibody (G-11) AC | sc-373816 AC | 500 µg/ml, 25% agarose | $424.00 | |||
IDH1/2 Antibody (G-11) HRP | sc-373816 HRP | 200 µg/ml | $322.00 | |||
IDH1/2 Antibody (G-11) FITC | sc-373816 FITC | 200 µg/ml | $336.00 | |||
IDH1/2 Antibody (G-11) PE | sc-373816 PE | 200 µg/ml | $349.00 | |||
IDH1/2 Antibody (G-11) Alexa Fluor® 488 | sc-373816 AF488 | 200 µg/ml | $364.00 | |||
IDH1/2 Antibody (G-11) Alexa Fluor® 546 | sc-373816 AF546 | 200 µg/ml | $364.00 | |||
IDH1/2 Antibody (G-11) Alexa Fluor® 594 | sc-373816 AF594 | 200 µg/ml | $364.00 | |||
IDH1/2 Antibody (G-11) Alexa Fluor® 647 | sc-373816 AF647 | 200 µg/ml | $364.00 | |||
IDH1/2 Antibody (G-11) Alexa Fluor® 680 | sc-373816 AF680 | 200 µg/ml | $364.00 | |||
IDH1/2 Antibody (G-11) Alexa Fluor® 790 | sc-373816 AF790 | 200 µg/ml | $364.00 | |||
IDH1/2 (G-11) Neutralizing Peptide | sc-373816 P | 100 µg/0.5 ml | $69.00 |