IDH1/2 Antibody (G-11): sc-373816

IDH1/2 Antibody (G-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects IDH1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). IDH1/2 (G-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IDH2 protein, also known as isocitrate dehydrogenase 2 (NADP+, mitochondrial), is a crucial enzyme composed of 452 amino acids, encoded by the human gene IDH2. IDH2 is integral to cellular metabolism, catalyzing the conversion of isocitrate to alpha-ketoglutarate while reducing NADP+ to NADPH, which maintains cellular redox state and supports glutathione synthesis, a key antioxidant. IDH2 functions are essential for energy production and intermediary metabolism, with dysregulation implicated in various metabolic disorders and cancers. IDH2 localizes in the mitochondrion, forming a homodimer and binding to either magnesium or manganese ions, which are critical for enzymatic activity. IDH2′s close association with the pyruvate dehydrogenase complex links carbohydrate metabolism to the tricarboxylic acid cycle, highlighting IDH2′s importance in cellular energy homeostasis.

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IDH1/2 Antibody (G-11) References:

1. Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.  |  Figueroa, ME., et al. 2010. Cancer Cell. 18: 553-67. PMID: 21130701
2. Isocitrate dehydrogenase 2 inhibits gastric cancer cell invasion via matrix metalloproteinase 7.  |  Wu, D. 2016. Tumour Biol. 37: 5225-30. PMID: 26553362
3. Enasidenib in mutant IDH2 relapsed or refractory acute myeloid leukemia.  |  Stein, EM., et al. 2017. Blood. 130: 722-731. PMID: 28588020
4. Isocitrate dehydrogenase type 2 (IDH2) is part of a multiprotein complex for placental steroidogenesis.  |  Urban-Sosa, VA., et al. 2019. Placenta. 87: 30-37. PMID: 31542634
5. The prognostic significance of wild-type isocitrate dehydrogenase 2 (IDH2) in breast cancer.  |  Aljohani, AI., et al. 2020. Breast Cancer Res Treat. 179: 79-90. PMID: 31599393
6. Isocitrate dehydrogenase 2 deficiency aggravates prolonged high-fat diet intake-induced hypertension.  |  Noh, MR., et al. 2020. Redox Biol. 34: 101548. PMID: 32388270
7. Therapy for isocitrate dehydrogenase 2 (IDH2)R172 -mutant acute myeloid leukaemia.  |  Linch, DC., et al. 2022. Br J Haematol. 196: 1348-1352. PMID: 34870324
8. IDH2 stabilizes HIF-1α-induced metabolic reprogramming and promotes chemoresistance in urothelial cancer.  |  Shigeta, K., et al. 2023. EMBO J. 42: e110620. PMID: 36637036
9. Wild-type IDH2 is a therapeutic target for triple-negative breast cancer.  |  Li, JJ., et al. 2024. Nat Commun. 15: 3445. PMID: 38658533
10. Epigenetic dysregulation in cancers by isocitrate dehydrogenase 2 (IDH2).  |  Nayarisseri, A., et al. 2024. Adv Protein Chem Struct Biol. 141: 223-253. PMID: 38960475

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IDH1/2 Antibody (G-11) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-IDH1/2 Antibody (G-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IDH1/2 Antibody (G-11) Specifications

Alternate Product Name: Isocitrate Dehydrogenase

Product Clone Name: G-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 123-154

Epitope Target: IDH2

Epitope Species: human

Product Reactivity/Detection: IDH1 and IDH2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for IDH1/2 Antibody (G-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred IDH2 antibody is IDH2 Antibody (B-6): sc-374476.