Date published: 2026-2-15

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IdeE Antibody (B-1): sc-390308

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Datasheets
  • IdeE Antibody (B-1) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
  • raised against amino acids 1-349 representing full length IdeE of Streptococcus equi subsp. zooepidemicus origin
  • recommended for detection of IdeE of S. equi strains H70, MCGS10565 and 4047 of Streptococcus equi origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for IdeE Antibody (B-1) for WB applications. These reagents are now offered in bundles with IdeE Antibody (B-1) (see ordering information below).
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    IdeE Antibody (B-1) is a mouse monoclonal IgG2a antibody that detects IdeE in Streptococcus equi by applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IdeE is a virulence factor produced by Streptococcus equi, playing a crucial role in the pathogen′s ability to evade the host′s immune response. Anti-IdeE antibody (B-1) functions by inhibiting the activation of the host′s complement system, which is a key component of the innate immune defense. By preventing complement activation, IdeE allows the bacteria to survive and proliferate within the host, leading to the severe inflammatory responses characteristic of strangles, a highly contagious disease primarily affecting young horses with underdeveloped immune systems. IdeE monoclonal antibody (B-1) is essential for researchers studying the mechanisms of S. equi pathogenesis and developing potential therapeutic strategies to combat strangles, making IdeE (B-1) antibody a valuable tool in equine health research.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    IdeE Antibody (B-1)

    sc-390308
    200 µg/ml
    $322.00

    IdeE Antibody (B-1): m-IgG Fc BP-HRP Bundle

    sc-540784
    200 µg Ab; 10 µg BP
    $361.00

    IdeE Antibody (B-1): m-IgG2a BP-HRP Bundle

    sc-546956
    200 µg Ab; 10 µg BP
    $361.00

    How much sample do you recommend loading on the gel for Western blot detection using IdeE (B-1): sc-390308?

    Asked by: Dr Ninau Qelp
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Satisfactory Western Blot data of IdeE expressionSatisfactory Western Blot data of IdeE expression in Streptococcus equi whole cell lysate. -SCBT QC
    Date published: 2013-06-02
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    IdeE Antibody (B-1) is rated 5.0 out of 5 by 1.
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