iASPP Antibody (A-2): sc-398566
- iASPP Antibody (A-2) is a mouse monoclonal IgG2a κ iASPP antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 107-395 mapping within an internal region of iASPP of human origin
- iASPP Antibody (A-2) is recommended for detection of iASPP of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-iASPP Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for iASPP Antibody (A-2) for WB applications. These reagents are now offered in bundles with iASPP Antibody (A-2) (see ordering information below).
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iASPP Antibody (A-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects iASPP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). iASPP (A-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. iASPP, a member of the apoptosis-stimulating protein of p53 (ASPP) family, plays a crucial role in regulating apoptosis through interaction with p53 tumor suppressor protein. While ASPP1 and ASPP2 enhance p53′s ability to activate pro-apoptotic genes, iASPP serves as a negative regulator, inhibiting p53′s pro-apoptotic functions. This inhibitory action is particularly significant as iASPP is the most evolutionarily conserved member of the ASPP family, and expression is notably upregulated in human breast carcinomas that retain wildtype p53. iASPP overexpression has been implicated in leukemogenesis and acute leukemia progression, making iASPP a potential target for therapeutic strategies aimed at tumors expressing wildtype p53. By inhibiting iASPP, researchers may find new avenues for cancer treatment, highlighting the importance of understanding regulatory mechanisms in apoptosis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
iASPP Antibody (A-2) References:
- Insulin-like growth factor-binding protein-3 modulates expression of Bax and Bcl-2 and potentiates p53-independent radiation-induced apoptosis in human breast cancer cells. | Butt, AJ., et al. 2000. J Biol Chem. 275: 39174-81. PMID: 10998426
- Down-regulation of X-linked inhibitor of apoptosis protein induces apoptosis in chemoresistant human ovarian cancer cells. | Sasaki, H., et al. 2000. Cancer Res. 60: 5659-66. PMID: 11059757
- ASPP proteins specifically stimulate the apoptotic function of p53. | Samuels-Lev, Y., et al. 2001. Mol Cell. 8: 781-94. PMID: 11684014
- The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization. | Slee, EA., et al. 2004. Oncogene. 23: 9007-16. PMID: 15489900
- The expression of iASPP in acute leukemias. | Zhang, X., et al. 2005. Leuk Res. 29: 179-83. PMID: 15607367
- Mdm2 and mdmX prevent ASPP1 and ASPP2 from stimulating p53 without targeting p53 for degradation. | Bergamaschi, D., et al. 2005. Oncogene. 24: 3836-41. PMID: 15782125
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
iASPP Antibody (A-2) | sc-398566 | 200 µg/ml | $322.00 | |||
iASPP Antibody (A-2): m-IgG Fc BP-HRP Bundle | sc-530842 | 200 µg Ab; 10 µg BP | $361.00 | |||
iASPP Antibody (A-2): m-IgGκ BP-HRP Bundle | sc-524389 | 200 µg Ab, 40 µg BP | $361.00 | |||
iASPP Antibody (A-2): m-IgG2a BP-HRP Bundle | sc-547609 | 200 µg Ab; 10 µg BP | $361.00 | |||
iASPP Antibody (A-2) AC | sc-398566 AC | 500 µg/ml, 25% agarose | $424.00 | |||
iASPP Antibody (A-2) HRP | sc-398566 HRP | 200 µg/ml | $322.00 | |||
iASPP Antibody (A-2) FITC | sc-398566 FITC | 200 µg/ml | $336.00 | |||
iASPP Antibody (A-2) PE | sc-398566 PE | 200 µg/ml | $349.00 | |||
iASPP Antibody (A-2) Alexa Fluor® 488 | sc-398566 AF488 | 200 µg/ml | $364.00 | |||
iASPP Antibody (A-2) Alexa Fluor® 546 | sc-398566 AF546 | 200 µg/ml | $364.00 | |||
iASPP Antibody (A-2) Alexa Fluor® 594 | sc-398566 AF594 | 200 µg/ml | $364.00 | |||
iASPP Antibody (A-2) Alexa Fluor® 647 | sc-398566 AF647 | 200 µg/ml | $364.00 | |||
iASPP Antibody (A-2) Alexa Fluor® 680 | sc-398566 AF680 | 200 µg/ml | $364.00 | |||
iASPP Antibody (A-2) Alexa Fluor® 790 | sc-398566 AF790 | 200 µg/ml | $364.00 |