Date published: 2026-5-25

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HYLS1 Antibody (E-3): sc-398834

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Datasheets
  • HYLS1 Antibody (E-3) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
  • raised against amino acids 1-299 representing full length HYLS1 of human origin
  • recommended for detection of HYLS1 of human origin by WB, IP, IF and ELISA
  • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HYLS1 Antibody (E-3) for WB applications. These reagents are now offered in bundles with HYLS1 Antibody (E-3) (see ordering information below).
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    HYLS1 Antibody (E-3) is a mouse monoclonal IgG2b antibody that detects hydrolethalus syndrome protein 1 (HYLS1) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HYLS1 is a highly conserved protein crucial for the formation and function of cilia, which are essential for various cellular processes, including signaling pathways and cellular motility. The proper functioning of cilia is vital for normal embryonic development and tissue homeostasis, as defects in cilia can lead to a range of developmental disorders and diseases, including hydrolethalus syndrome, characterized by severe malformations. The gene encoding HYLS1 is located on human chromosome 11, a region known for its density of gene and disease associations, highlighting the importance of this chromosome in human health. Additionally, HYLS1 interacts with several other proteins involved in ciliary assembly and function, further underscoring its role in maintaining cellular integrity and signaling. Anti-HYLS1 antibody (E-3) effectively detects this critical protein, making HYLS1 (E-3) antibody an invaluable tool for researchers studying cilia-related disorders and developmental biology.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    HYLS1 Antibody (E-3)

    sc-398834
    200 µg/ml
    $322.00

    HYLS1 Antibody (E-3): m-IgGκ BP-HRP Bundle

    sc-536159
    200 µg Ab; 40 µg BP
    $361.00

    HYLS1 Antibody (E-3): m-IgG2b BP-HRP Bundle

    sc-549520
    200 µg Ab; 10 µg BP
    $361.00

    How much sample do you recommend loading on the gel for Western blot detection using HYLS1 (E-3): sc-398834?

    Asked by: Dr Ninau Qelp
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Produced positive Western blot data of HYLS1Produced positive Western blot data of HYLS1 expression in non-transfected and human HYLS1 transfected 293 whole cell lysates. -SCBT QC
    Date published: 2013-08-27
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    HYLS1 Antibody (E-3) is rated 5.0 out of 5 by 1.
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