HuR Antibody (3A2) recommended for detection of HuR, HuB, HuC and HuD of mouse, rat, human and x origin by WB, IP, IF, IHC(P) and ELISA

HuR Antibody (3A2): sc-5261

HuR Antibody (3A2) is rated 4.6 out of 5 by 23.
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Datasheets

    • Anti-HuR Antibody (3A2) is a mouse monoclonal IgG1 (kappa light chain) HuR antibody provided at 200 µg/ml
    • raised against full length HuR of human origin
    • HuR Antibody (3A2) is recommended for detection of HuR, HuB, HuC and HuD of mouse, rat, human and x origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-HuR Antibody (3A2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HuR (3A2): sc-5261.
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HELLO,I want to do RIP and WB experiment in mouse,which antibody should I choose?HuR Antibody (3A2) or HuR Antibody (3A2) AC.?

Asked by: ncestt
Thank you for your question. If you need to use the same antibody for both Western blotting and immunoprecipitation, then we would recommend purchasing the unconjugated primary antibody, sc-5261: HuR (3A2). The ‘AC’ represents the primary antibody directly conjugated to agarose beads, and is only suitable for immunoprecipitation experiments.
Answered by: Technical Support
Date published: 2019-07-13

Is this antibody works well for detecting use flow cytometry?

Asked by: Yong w
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-01-17

I HAVE A QUESTION ABOUT THIS ANTIBODY. IT IS POSSIBLE TO REACT POSITIVELY AGAINST GST-FUSION PROTEINS?.

Asked by: FLOPITZOTSOA
Thank you for your question. This monoclonal antibody, HuR (3A2): sc-5261, is recommended for detection of HuR, HuB, HuC and HuD of mouse, rat, human and xenopus origin. The antibody is not recommended for detection of GST. However, the antibody is suitable for detection of GST-HuR, HuB, HuC or HuD fusion proteins.
Answered by: Technical Support
Date published: 2017-11-15

Hello, for research only I need to know whether this antibody (HuR (3A2): sc-5261) binds C-terminal or N-terminal HuR?

Asked by: Theresa Liebe
Thank you for your question. HuR Antibody (3A2): sc-5261 is raised against full length recombinant HuR of human origin, and we have not determined the exact epitope recognized by this mouse monoclonal antibody. According to the literature (PMID: 10737787), “the 3A2 epitope is located in RRM 1 (RNA Recognition Motif 1) of HuR, which is highly conserved among all ELAV proteins [82% identity between HuR, HuD, and Hel-N1/N2, and 72% identity between HuR and HuC].”
Answered by: Technical Support
Date published: 2017-06-08

Does this antibody work well with CLIP?

Asked by: beachbum
Thank you for your question. We have not tested this antibody for CLIP, and are not aware of any publications citing use of the antibody for that application. HuR Antibody (3A2): sc-5261 is a mouse monoclonal that has been cited in over 25 publications for immunoprecipitation (IP). Based on the antibody's performance in that application and the similarity with CLIP, it may also be suitable for that application, but that would have to be determined experimentally.
Answered by: Technical Support
Date published: 2017-03-10

What is the epitope of HuR Antibody (3A2): sc-5261?

Asked by: Dr Ninau Qelp
HuR Antibody (3A2): sc-5261 was raised against full length HuR of human origin. We have not mapped the epitope.
Answered by: Tech Service 2
Date published: 2016-12-15

Can HuR Antibody (3A2): sc-5261 be used for bovine samples?

Asked by: Dr Ninau Qelp
Yes. We have several publications that cite using HuR Antibody (3A2): sc-5261 in bovine samples. Please see PMID: 18206386, 20018854, and 22879735
Answered by: Tech Service 2
Date published: 2016-12-15
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Rated 1 out of 5 by from This review is for C23 Antibody (MS-3). C23 Antibody (MS-3) (Lot #H0818), which I purchased Nov. 2018, is not detecting any signal in BT20 breast and Hey ovarian cancer cells. I did IB multiple times with no success.
Date published: 2019-02-06
Rated 4 out of 5 by from review of sc-5261 The anti-HuR 3A2 works very well in immunofluorescence
Date published: 2018-07-27
Rated 5 out of 5 by from HuR Antibody (3A2) HuR Antibody (3A2) works good in western blot from Cytosol fractions.
Date published: 2018-03-08
Rated 5 out of 5 by from excellent worked perfectly in western blot on rat tissue samples
Date published: 2017-12-13
Rated 5 out of 5 by from Nice antibody for WB I have tried this antibody in western blot. It detects a strong band at the predicted size of HuR.
Date published: 2017-11-21
Rated 4 out of 5 by from a good product i bought the antibody a month ago,which have significant effect in the following experiments.And it have a great reactivity with rat cardiac Myocytes.
Date published: 2017-11-21
Rated 5 out of 5 by from Great Antibody for sensitive HuR detection This antibody works very well for me in detecting HuR as a single band from my HeLa whole cell lysates and RNA affinity purifications. The sensitivity is very good.
Date published: 2017-11-15
Rated 5 out of 5 by from Good signal Robust bands seen in WB using 3T3-L1 whole cell lysates, happy with thew product
Date published: 2017-09-01
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